微生物来源的β-甘露聚糖酶的研究与应用进展

Study and Application of Microbialβ-mannanases

甘露聚糖是软木半纤维素的主要组成成分,广泛存在于植物组织中。β-甘露聚糖酶是最关键的甘露聚糖降解酶,以内切方式催化甘露聚糖中β-1,4-连接的甘露糖苷键的水解。β-甘露聚糖酶在植物、动物、真菌、细菌和放线菌中均存在。微生物来源的β-甘露聚糖酶能在较宽的pH和温度范围中发挥催化活性,因此在食品、制药、饲料、造纸、生物炼制等行业具有广泛的应用前景。从β-甘露聚糖酶的微生物来源、分类、酶学性质、分子改造和实际应用等方面进行综述,并对微生物来源的β-甘露聚糖酶的未来研究方向进行展望,为微生物β-甘露聚糖酶的研究与应用提供理论基础。

Mannan is the main component of cork hemicellulose and widely exists in plant tissues.β-Mannanase is the most critical mannan-degrading enzyme,which catalyzes the hydrolysis ofβ-1,4-linked mannosidic bonds in mannans in an endo-acting manner.β-Mannanases are found in plants,animals,bacteria,fungi and actinomycetes.Microbial derivedβ-mannanase can exert catalytic activity in a wide range of pH and temperature,so it has a wide range of application prospects in food,pharmaceutical,feed,paper,biorefinery and other industries.In the present article,the microbial origin,classification,enzymatic properties,molecular modification and practical application ofβ-mannanases were reviewed,the future research direction of microbialβ-mannanases was prospected.This review will provide a theoretical basis for the research and application of microbialβ-mannanase.