健脾补肾法对人结直肠癌移植瘤组织TLR4、RAGE、NF-κB及CD34、D2-40的影响

Effects of Spleen-and-Kidney Invigorating Method on TLR4,RAGE,NF-κB,CD34,D2-40 in Human Colorectal Cancer Transplanted Tumor Tissue

目的探讨健脾补肾法对人结直肠癌移植瘤组织TLR4、RAGE、NF-κB及CD34、D2-40的影响。方法于裸鼠右腿内侧接种人结肠癌细胞,建立人结肠癌裸鼠皮下移植瘤模型,15d后脱颈处死剥离瘤体。对照组用生理盐水灌胃,实验组用健脾补肾中药灌胃,免疫组化法检测2组移植瘤组织中TLR4、RAGE、NF-κB及CD34、D2-40的表达。结果免疫组化显示,与对照组相比,实验组的移植瘤组织中TLR4、RAGE、NF-κB的表达均明显下调(P<0.05),微血管CD34及淋巴管D2-40也明显下调(P<0.005)。结论健脾补肾法能明显下调人结直肠癌移植瘤组织炎症受体TLR4及RAGE、核转录因子NF-κB、肿瘤新生血管标记物CD34及肿瘤新生淋巴管标记物D2-40的表达。

To study the effect of spleen-and-kidney invigorating method on TLR4,RAGE,NF-κB,CD34,D2-40 in human colorectal cancer transplanted tumor tissue.Methods:Human colorectal cancer cells were inoculated on the inside of the right leg of nude mice to establish a subcutaneous tumor transplantation model of human colorectal cancer in nude mice and the mice was sacrificed to remove the tumor from the neck 15 days later.The control group was given intragastric administration with normal salt and the experimental group was intragastrically given with decoction for invigorating spleen and kidney.The expressions of TLR4,RAGE,NF-κB,CD34 and D2-40 in the transplanted tumor tissues were detected by immunohistochemical method.Results:Compared with that of the control group,the expressions of TLR4,RAGE and NF-κB in the experimental group were significantly down-regulated(P<0.05),and the expressions of CD34 in microvessels and D2-40 in lymphatic vessels were also significantly down-regulated(P<0.005).Conclusion:The spleen-and-kidney invigorating method can significantly down-regulate the expression of inflammatory receptor TLR4 and RAGE,nuclear transcription factor NF-κB,tumor neovascularization marker CD34 and tumor neovascularization marker D2-40 in human colorectal cancer graft tissue.