天山南麓绵羊沙门菌耐药基因检测及耐药性分析

Detection and drug resistance analysis of Salmonella resistance genes in sheep in southern Tianshan Mountains

为了研究天山南麓地区绵羊沙门菌(Salmonella)耐药性及耐药基因的流行现状,本试验从天山南麓地区选择10个采样点采集绵羊粪样进行沙门菌分离鉴定,采用K-B药敏纸片法进行药敏试验,并通过PCR法检测耐药基因。结果表明:共分离出67株沙门菌,分离株对青霉素、磺胺异噁唑耐药率较高,分别为98.51%、68.66%,其次为氨苄西林、链霉素、阿莫西林、四环素,耐药率分别为49.25%、47.76%、46.27%和43.28%,分离株多重耐药率为55.22%。分离株耐药基因中KN和sul1检出率最高,分别为100.00%和98.51%,aadA1、aph(3')-IIa、TEM、ant(3'')-Ia和CTX-M的检出率均在50%以上,分别为82.09%、71.64%、62.69%、58.21%和50.75%。药敏试验和耐药基因检测结果的综合分析表明天山南麓地区沙门菌的耐药性及耐药基因流行状况较为严重,呈区域性流行。

In order to study the epidemic status of Salmonella resistance and antibiotic resistance genes of sheep in the southern foothill of Tianshan Mountains,ten samples of sheep feces were selected from the area for Salmonella separation and identification,and K-B method was used for drug susceptibility test.Drug resistance genes were detected by PCR.The results showed that 67 strains of Salmonella were isolated,and the drug resistance rates to penicillin and sulfaoxazole were 98.51%and 68.66%,respectively,followed by ampicillin,streptomycin,amoxicillin and tetracycline were 49.25%,47.76%,46.27%and 43.28%,respectively.The multiple drug resistance rate of isolates was 55.22%.The detection rates of KN and sul1 were 100.00%and 98.51%,respectively,followed by aadA1,aph(3')-IIa,TEM,ant(3'')-Ia and CTX-M were all above 50%,which were 82.09%,71.64%,62.69%,58.21%and 50.75%,respectively.The comprehensive analysis of drug susceptibility test and drug resistance gene test results showed that the prevalence of drug resistance and drug resistance gene of Salmonella was serious in the southern foothills of Tianshan Mountains,showing a regional epidemic.