基于GC-IMS的不同产地烟草中挥发性风味物质分析

Analysis of volatile components in tobacco from different regions based on GC-IMS

采用气相色谱-离子迁移色谱(GC-IMS)技术对贵州遵义、福建三明、湖南永州3个产地烟草中挥发性风味物质进行鉴定,结合主成分分析探究3个产地烟草之间挥发性风味物质的差异,并根据该差异对3个产地烟草进行区分。结果表明:GC-IMS共检测到125种挥发性风味物质,其中已定性79种,醛类、酮类、醇类、酯类是3个产地烟草的主要挥发性风味物质;3个产地烟草样品中醛类物质是整体风味的主要贡献者,但在种类和含量上存在较大差异,且不同产地样品中的挥发性风味物质分布分别占据了相对独立的空间。与电子鼻技术相比,GC-IMS技术能够建立3个产地烟草挥发性风味物质指纹图谱,可视化呈现3个产地烟草中挥发性风味物质的轮廓,达到对烟草进行鉴别及产地溯源的目的。

The volatile flavor compounds in tobacco from different regions(Zunyi,Sanming and Yongzhou)were identified by gas chromatography-ion mobility spectrometry(GC-IMS).The differences of volatile flavor compounds among the three regions were investigated by principal component analysis,and the tobacco from the three regions was distinguished according to the differences.The results showed that 125 volatile flavor compounds were detected by GC-IMS,of which 79 were identified.Aldehydes,ketones,alcohols and esters were the main volatile compounds in the three regions.Aldehydes in tobacco samples from the three regions were the main contributors to the overall flavor,but there were great differences in the types and contents,and the distribution of volatile flavor substances in samples from different regions occupied relatively independent space.Compared with electronic nose technology,GC-IMS method can establish the fingerprint of volatile flavor substances in tobacco from three regions and visually present the outline of volatile flavor substances in tobacco from three regions,so as to achieve the purpose of identification and origin traceability of tobacco.