透明质酸-大豆β-伴球蛋白复合膜对鲢鱼片微冻贮藏品质影响

Effect of Hyaluronic Acid-β-Conglycinin Composite Film on Quality ofSilver Carp Fillet During Preservation by Partial Freezing

为解决淡水鱼在贮藏过程中蛋白质氧化及品质劣变问题,采用0.9%透明质酸(hyaluronic acid,HA)分别与1%、2%、3%大豆β-伴球蛋白制备复合涂膜剂处理鲢鱼肉,在-3℃微冻贮藏28 d,考察鲢鱼肉贮藏期的持水力、色差(△E)、pH值、电导率、挥发性盐基氮(total volatile basicnitrogen,TVB-N)、硫代巴比妥酸值(thiobarbituric reactive subs-tances,TBARS)、三氯乙酸(trichloroacetic acid,TCA)可溶性肽含量的变化,探究鱼肉肌原纤维蛋白氧化规律,结合主成分分析(principal component analysis,PCA)研究涂膜及微冻技术对鲢鱼肉蛋白质氧化及品质的控制作用。结果表明:与未经涂膜鲢鱼肉相比,复合涂膜可以延缓鲢鱼肉TVB-N、TBARS、pH值、电导率、TCA可溶性肽含量的升高,抑制鱼肉持水力、△E的下降。经复合涂膜处理,鱼肉肌原纤维蛋白巯基含量及钙离子ATP酶(calcium-ATPase,Ca^(2+)-ATPase)的活性提高,羰基含量和表面疏水性明显降低。主成分分析提取的2个主成分的累计方差贡献率达到86.782%,能较好地反映原始信息,可直观了解到各涂膜处理鲢鱼肉品质变化趋势。综合评定,0.9%HA-2%大豆β-伴球蛋白涂膜鱼肉的保鲜效果最佳。

To solve the problem of protein oxidation and quality deterioration of freshwater fish during storage,this paper used 0.9%hyaluronic acid(HA)and 1%,2%and 3%β-conglycinin to prepare a composite coating film for silver carp fillet,preserved the fillet at-3℃for 28 d,and investigated the changes in water-holding capacity,whiteness(△E),pH,conductivity,total volatile basicnitrogen(TVB-N),thiobarbituric reactive substances(TBARS),and trichloroacetic acid(TCA)-soluble peptides of silver carp fillet,thereby exploring the oxidation rule of fish myofibrillar proteins during the storage.The principal component analysis(PCA)was combined to study the control effect of coating film and partial-freezing technology on the oxidation and quality of silver carp protein.The results showed that the composite coating film delayed the increase of TVB-N,TBARS,pH,conductivity,and TCA-soluble peptide content,and inhibited the decrease of water holding and △E of the fish.The sulfhydryl content in fish myofibrillar proteins and Ca^(2+)-ATPase activity was increased by composite coating,whereas the carbonyl content and surface hydrophobicity were significantly reduced.The cumulative variance contribution rate of the two principal components extracted by PCA reached 86.782%,which well reflected the original information to understand the change trend of the quality of silver carp with different film treatments.According to the comprehensive assessment,the silver carp coated with 0.9%HA-2% β-conglycinin had the optimal preservation effect.