柴胡疏肝散精简方对抑郁大鼠行为学及中缝核内色氨酸代谢的影响

Effects of Simplified Chaihu Shugan Powder on Behaviors and Regulating the Metabolism of Tryptophan in Raphe Nucleus of Depressive Rats

目的观察柴胡疏肝散精简方对抑郁大鼠行为学的影响,探讨柴胡疏肝散精简方可能存在的抗抑郁机制。方法采用腹腔注射利血平建立大鼠抑郁模型,以盐酸氟西汀为阳性对照药,将32只SD雄性大鼠随机分为空白组、模型组、氟西汀组、精简方组,每组8只,各药物组灌胃相应药物,连续14 d。观察大鼠一般情况行为学,检测大鼠中缝核内5-羟色胺(5-HT)、色氨酸羟化酶2(TPH2)、吲哚胺2,3双加氧酶(IDO)的含量。结果与空白组比较,模型组体质量增加、糖水偏好指数、旷场实验运动总距离明显减少(P<0.01);中缝核内TPH2、5-HT含量明显减少(P<0.01),IDO含量明显增加(P<0.01)。与模型组比较,精简方组体质量增加、旷场实验运动总距离明显增加(P<0.01);糖水偏好指数增加(P<0.05);中缝核内TPH2、5-HT含量明显增加(P<0.01),IDO含量明显减少(P<0.01)。结论柴胡疏肝散精简方可能通过增加中缝核内TPH2含量(促进色氨酸-5羟色胺途径),降低IDO的含量(抑制色氨酸-犬尿氨酸途径),进而使中缝核内5-HT含量增加,发挥抗抑郁作用。

Objective To observe the effect of Simplified Chaihu Shugan Powder(SCSP)on behaviors,to explore the mechanism of the effect of Simplified Chaihu Shugan Powder.Method Reserpine were intraperitoneal injected for 14 days to establish depressive models.Fluoxetine was used as the positive control drug,32 male SD rats were randomly divided into control group,model group,fluoxetine group,SCSP group,each group of 8 rats.Meanwhile,the fluoxetine group and SCSP group were treated with fluoxetine or SCSP.At the end of the experiment,we use behavioral experiment to evaluate the depressive status.The contents of 5-HT,TPH2,IDO were detected in raphe nucleus.Result Compared with the control group,the body weight gain,sugar water preference index,total distance of open field experiment in model group decreased significantly(P<0.01);the contents of TPH2 and 5-HT in raphe nucleus decreased significantly(P<0.01),the contents of IDO in raphe nucleus increased significantly(P<0.01)in model group.Compared with the model group,the body weight gain,total distance of open field experiment in SCSP group increased significantly(P<0.01);the sugar water preference index increased(P<0.05)in SCSP group;the contents of TPH2 and 5-HT in raphe nucleus increased significantly(P<0.01),the contents of IDO in raphe nucleus decreased significantly(P<0.01)in model group.Conclusion SCSP has certain function of anti-depression of depressive rats caused by reserpine.It may increase the content of TPH2 in the raphe nucleus(promote the tryptophan-5-hydroxytryptophan pathway),reduce the content of IDO(inhibit the tryptophan-canine urine pathway),and then increase the content of 5-HT in the raphe nucleus.