C57BL/6J超排小鼠取卵时间对体外受精效果的影响

ect of Collecting Oocyte on Vitrofertilization in C57BL/6J Superovulated Mic

目的探讨雌性小鼠注射绒毛膜促性腺激素(HCG)后,取卵时间对体外受精率的影响。方法选用3~4周龄的野生型C57BL/6J雌鼠,体质量10~13 g,通过腹腔注射血清促性腺激素(PMSG)和HCG联合使用[1]做超排处理。我们将超排后雌鼠取卵时间分为6个时间段,分别为13、14、15、16、17、18 h后取卵母细胞与新鲜精子做体外受精。取3月龄雄性小鼠附睾里精子,在HTF液里获能1 h后,用于体外受精。实验共分3组,每组12只雌鼠用于超排处理,共得到2-细胞胚胎数分别为258、199和243枚;分别移植到当天见栓的假孕鼠输卵管内,得到出生仔鼠分别为98只、87只、95只;出生率分别为37.98%、43.72%和39.09%。结果总取卵数和2-细胞发育率,超排后15 h取卵发育受精率最高,这说明小鼠卵母细胞超排后14 h少数成熟,15~16 h完全成熟,排卵17 h后则开始出现退化。结论3组实验最高受精率比较:第1组15 h后取的卵母细胞团受精率最高为79.17%、第2组15 h后取的卵母细胞团受精率最高为75.68%、第3组16 h后取的卵母细胞团受精率最高为76.92%。

Collecting Oocyte time on IVF rate after HCG injection.Method 3-4 week-old wild-type C57BL/6J females with 10-13 g were treated with PMSG and HCG.We divided the egg retrieval time of female mice after superarrangement into six periods,after 13,14,15,16,17 and 18 h for in vitro fertilization.Sperm from the epididymis of 3-month-old male mice were recovered in HTF fluid for 1 h and then used for in vitro fertilization.The experiment was divided into 3 groups,and 12 females were used for overarrangement,and 258,199 and 243 embryos were obtained.In the experiment,98,87 and 95 births were obtained;the birth rates were 37.98%,43.72%and 39.09%respectively.Result The total number of eggs and the development rate of 2 cells were the highest at 15 h after superovulation,which showed that the mouse oocytes matured after 14 h,fully mature at 15 to 16 h,and began to degenerate after 17 h of ovulation.Conclusion Comparison of the highest fertilization rate in the 3 groups:the oocyte mass fertilization rate after 15 h in the first group was 79.17%,the oocyte mass fertilization rate in the second group after 15 h was 75.68%,and the oocyte mass fertilization rate after 16 h in the third group was 76.92%.