不同退行性视网膜病变动物间眼轴长度的比较

Comparison of Ocular Axial Length between Animals with Different Degenerative Retinopathy

目的比较同一体质量区间不同品系大鼠、小鼠眼轴的差别。方法选用野生型(SD)大鼠、实验室现有的不同品系大鼠和小鼠:先天性静止性夜盲(CSNB)大鼠、卷毛(HL)大鼠、视网膜锥细胞失功能(RCD)大鼠、C57BL/6小鼠、锥体光感受器功能丧失1(cpfl1)小鼠、锥体光感受器功能丧失3(cpfl3)小鼠、先天性静止性夜盲(Nob2)小鼠,按照体质量划分为不同组别(大鼠以50 g为一区间分为10组,小鼠以10 g为一区间共分为两组),测量其眼轴,在同一体质量区间比较不同品系大鼠和小鼠之间的眼轴区别。结果大鼠与小鼠眼轴长度随体质量增加而增长,在不同品系之间存在差异。其中100~150 g体质量的CSNB大鼠眼轴长度大于SD大鼠(P<0.05)。150~200 g体质量的RCD大鼠眼轴长度大于SD大鼠(P<0.01),但SD与CSNB大鼠之间不具有统计学差异;200~250 g、450~500 g、500~550 g体质量的HL与RCD大鼠眼轴较SD大鼠长(P<0.05),但SD与CSNB大鼠之间没有统计差异;250~300 g与300~350 g体质量的三种品系大鼠眼轴均长于SD大鼠(P<0.01);350~400 g体质量的CSNB与RCD大鼠眼轴长于SD大鼠(P<0.01),但SD大鼠与HL大鼠之间无统计学差异;400~450 g体质量的四种品系大鼠眼轴均无统计学差异;550~600 g体质量的HL大鼠眼轴较SD大鼠长(P<0.05),CSNB与SD大鼠之间无统计差异。全部体质量的C57BL/6小鼠眼轴长度短于其余3个品系的小鼠(P<0.01);结论不同体质量区间,各基因突变大鼠或小鼠眼轴长于对照鼠(SD大鼠C57BL/6小鼠)眼轴,且在大鼠中,此差异在发育期更为明显。

Objective To compare the difference of ocular axial length of different strains of rats and mice in the same weight range.Method Wild-type Sprague Dawley rats and different strains of mice existing in the laboratory were selected:Congenital stationary night blindness model rats(CSNB rats),HL rats,Retinal cone dysfunction model rats(RCD rats),C57BL/6 mice,Cone photoreceptor function loss 1(cpfl1),cone photoreceptor function loss3(cpfl3),Congenital stationary night blindness model mice(Nob2 mice).The rats were divided into different groups according to their body weight,and their ocular axial length were measured.The differences of ocular axial length between rats and mice of different strains were compared in the same weight range.Result The ocular axial length of eye increased with the increase of body weight in rats and mice,and there were differences among different strains.The axial length of CSNB rats in the body weight range of 100-150 g was larger than that of SD rats(P<0.05).The axial length of the eyes of RCD rats with 150-200 g body weight was larger than that of SD rats(P<0.01),but there was no statistical difference between SD and CSNB rats.The ocular axis of HL and RCD rats with body weight of 200-250 g,450-500 g and 500-550 g was longer than that of SD rats(P<0.05),but there was no statistical difference between SD and CSNB rats.The axial length of eyes of 250-300 g and 300-350 g strains was longer than that of SD rats(P<0.01).The axial length of CSNB and RCD rats at 350-400 g body weight was longer than that of SD rats(P<0.01),but there was no statistical difference between SD rats and HL rats.There was no statistical difference in ocular axis between 400-450 g weight and four strains of rats.The ocular axis of HL rats was longer than SD rats at 550-600 g body weight(P<0.05),and there was no statistical difference between CSNB and SD rats.The axial eye length of C57BL/6 mice with full body weight was shorter than that of the other three strains(P<0.01).Conclusion The length of the eye axis in the mutant rats or mice was longer than that in the control rats(SD rats C57BL/6 mice)at different body weight ranges,and the difference was more obvious in the development period of the rats.