受精卵动态形成参数时间与胚胎发育潜能相关性研究

Study on Correlation between Parameters Time of Dynamic Formation of Fertilized Egg and Embryonic Development Potential

目的:探讨受精卵关键事件发生时间与形成优质卵裂期胚胎(优胚)之间是否有相关性。方法:选取佛山市妇幼保健院2021年2月至2021年9月进行体外受精辅助治疗的患者作为研究对象。根据是否形成优胚分为:未形成优胚组724例,形成优胚组476例。比较两组之间关键事件发生时间差异及其时间与是否形成优胚的相关性。结果:两组患者的受精卵雌雄原核同步形成时间(tPNa)、雌雄原核相接触时间(tPC)、雌雄双原核同步形成时间与双原核相接触的时间间隔(T1)及雌雄双原核同步消失与受精卵发生首次卵裂的时间间隔(T2)比较,差异均无统计学意义(P>0.05);形成优胚组的雌雄原核消失时间(tPNf)较未形成优胚组更短,差异具有统计学意义(P<0.05)。但相关性分析结果显示,tPNa、tPC、tPNf、T1、T2与优胚形成均不存在相关性。结论:受精卵关键事件发生时间与优胚的形成之间并不存在相关性。

Objective To investigate whether there is correlation between the occurrence time of key events in fertilized eggs and the formation of high-quality cleavage embryos.Methods Patients receiving in vitro fertilization(IVF)assisted treatment in Foshan Maternal and Child Health Hospital from February 2021 to September 2021 were selected as the research objects.According to the formation of good embryos,there were 724 cases in the group without good embryos and 476 cases in the group with good embryos.To compare the difference of critical event occurrence time between the two groups and its correlation with the formation of embryos.Results There were no significant differences in the synchrony formation time of male and female prokaryotes(tPNa),the contact time of male and female prokaryotes(tPC),the time interval between the synchrony formation time of male and female diprokaryotes and the contact time of diprokaryotes(T1),and the time interval between the disappearance of synchrony between male and female diprokaryotes and the first cleavage of fertilized eggs(T2)between the two groups(P>0.05).The time of pronucleus fading(tPNf)of male and female prokaryotes in the optimal embryo group was shorter than that in the non-optimal embryo group,and the difference was statistically significant(P<0.05).However,correlation analysis showed that tPNa,tPC,tPNf,T1 and T2 were not correlated with the formation of embryos.Conclusion There was no correlation between the time of critical events in fertilized eggs and the formation of high-quality embryos.