基于网络药理学探讨苦参治疗阴道炎的作用机制

Mechanism of Kushen (Radix Sophorae Flavescentis) in the Treatment of Vaginitis Based on Network Pharmacology

目的:通过网络药理学方法研究苦参治疗阴道炎的潜在作用靶点及作用机制。方法:运用中药系统药理数据库及分析平台(TCMSP)筛选苦参有效成分及靶点,运用人类孟德尔遗传病数据库(OMIM)、GeneCards数据库收集阴道炎相关靶点。通过微生信筛选出共同靶点,运用String数据库和Cytoscape 3.7.0软件分析蛋白互作关系(PPI)。运用Metascape数据库进行基因本体(GO)生物过程富集分析和京都基因和基因组百科全书(KEGG)代谢通路富集分析,并运用微生信做富集气泡图,最后利用Cytoscape 3.7.0软件作“苦参有效成分-阴道炎-靶点-KEGG”网络构建。结果:苦参可能通过白细胞介素17(IL17)及核转录因子-κB(NF-κB)信号通路对阴道炎发挥治疗作用,作用靶点包括丝裂原活化蛋白激酶1(MAPK1)、血管内皮生长因子(VEGFA)、白细胞介素6(IL6)、基质金属蛋白酶9(MMP9)、基质金属蛋白酶2(MMP2)、白细胞介素4(IL4)以及白细胞介素2(IL2)等。结论:苦参可能作用于IL17及NF-κB信号通路中的MAPK1、VEGFA、IL6、MMP9、MMP2、IL4、IL2靶点而治疗阴道炎。

Objective: To analyze the potential mechanism of Kushen(Radix Sophorae Flavescentis) in the treatment of vaginitis by means of network pharmacology. Methods: The effective components and targets of Kushen were screened by TCMSP database. The relevant targets of vaginitis were collected by OMIM and GeneCards database. The common targets were screened out by Micro Channel. The relationship of protein-protein interaction was analyzed by the String database and Cytoscape 3.7.0 software. The gene ontology(GO), kyoto encyclopedia of genes and genomes(KEGG) metabolic pathway were analysed using Metascape database. The enrichment bubble diagram was worked by Micro Channel. Finally, the network of “active ingredient of Kushen-vaginitis-target-KEGG” was constructed by Cytoscape 3.7.0 software. Results: Kushen may play a therapeutic role in vaginitis through IL17 and NF-κB signaling pathway. The targets include MAPK1, VEGFA, IL6, MMP9, MMP2, IL4, IL2, etc. Conclusion: Kushen may put therapeutic effect on vaginitis dependent on IL17 and NF-κB signaling pathway via targeting MAPK1, VEGFA, IL6, MMP9, MMP2, IL4 and IL2.