海藻多糖中硫酸基含量对抑制草酸钙晶体形成和修复受损伤肾小管上皮细胞的影响

Effect of seaweed polysaccharides with different sulfate group contents on crystal growth of calcium oxalate and on the repair of damaged renal epithelial cells

采用X射线衍射法、傅里叶变换红外光谱仪、扫描电子显微镜、ζ电位仪和电感耦合等离子体发射光谱仪,比较了4种分子量相近(约3700 Da)、但硫酸基(—OSO_(3)-)含量不同的降解海藻多糖对草酸钙(CaC2O_(4))晶体生长的调控作用;采用细胞实验比较了它们对受损伤肾小管上皮细胞(HK-2)的修复能力。这4种海藻多糖分别是降解紫菜多糖(PY-1)、降解龙须菜多糖(GL-2)、降解羊栖菜多糖(SF-3)和降解裙带菜多糖(UP-4),其—OSO_(3)-含量分别为17.9%、13.3%、8.2%和5.5%。结果表明,这4种多糖均能够抑制一水草酸钙(COM)生长,诱导二水草酸钙(COD)生成,增加溶液中可溶性Ca2+离子浓度,增加晶体表面的ζ电位绝对值,从而降低晶体的聚集程度;这些多糖均能修复被草酸氧化损伤的HK-2细胞,提高细胞活力和超氧化物歧化酶(SOD)活力,降低丙二醛(MDA)的释放量。结果表明,多糖中—OSO_(3)-含量越高,多糖对CaC2O_(4)晶体生长的抑制作用和对受损伤HK-2细胞的修复作用均越强。

X-ray diffraction,Fourier transform infrared spectrometer,scanning electron microscope,ζpotential analyzer,and inductively coupled plasma emission spectrometer were used to compare the regulation of four degraded seaweed polysaccharides with a similar molecular weight of about 3700 Da and different contents of sulfate group(—OSO_(3)-)on the inhibition of crystal growth of calcium oxalate(CaC2O_(4)),and the repair ability of the polysaccharides on damaged renal epithelial cells was compared by cell experiments.These four seaweed polysaccharides were degraded Porphyra polysaccharide(PY-1),degraded Gracilaria lemaneiformis polysaccharide(GL-2),degraded Sargassum fusiforme polysaccharide(SF-3)and degraded Undaria pinnatifida polysaccharide(UP-4),and their—OSO_(3)-contents were 17.9%,13.3%,8.2%,and 5.5%,respectively.The results showed that these four polysaccharides could inhibit the growth of calcium oxalate monohydrate(COM),induce the formation of calcium oxalate dihydrate(COD),increase the concentration of soluble Ca2+ions in solution,and increase the absolute value ofζpotential on the crystal surface,thereby reducing the degree of crystal aggregation.In addition,these polysaccharides could repair HK-2 cells damaged by oxalic acid,increase cell viability and superoxide dismutase(SOD)activity,and reduce the release of malondialdehyde(MDA).The results show that the higher the content of—OSO_(3)-in polysaccharides,the stronger the inhibitory effect of polysaccharides on CaC2O_(4) crystal growth and the repair effect on damaged HK-2 cells.