玉米高赖氨酸和糯性基因聚合材料营养品质分析

Nutritious Quality Analysis of Materials with Pyramiding High Lysine and Waxy Mutant Genes in Maize

为评价玉米高赖氨酸和糯性基因聚合材料的营养品质,本研究以玉米高赖氨酸(o2、o16)和糯性(wx)基因不同聚合类型材料及其亲本为试材,利用近红外分析仪和全自动氨基酸分析仪对籽粒油分含量、总淀粉含量、蛋白质含量和氨基酸含量等指标进行分析。结果表明,与轮回亲本CML 530相比,o2、o16和wx三基因聚合材料,W 1(o2o16wx)籽粒油分含量降低了20.69%,总淀粉含量提高了4.22%,赖氨酸含量提高了72.57%。4份o2和o16两基因聚合材料,W 2(o2o16)、W 3(o2o16)、W 11(o2o16)和W 12(o2o16)籽粒总淀粉含量平均提高了2.92%;赖氨酸含量分别提高了68.55%、67.68%、49.77%、56.08%。7份o16和wx两基因聚合的材料中,W 4(o16wx)和W 5(o16wx)籽粒蛋白质含量平均增加了8.10%;W 6(o16wx)、W 7(o16wx)和W 9(o16wx)籽粒赖氨酸含量分别增加了9.84%、12.30%和8.66%。可见,这些优质基因聚合材料是品质性状遗传改良和育种应用的重要材料。

To evaluate nutritional quality of materials with pyramiding high lysine and waxy mutant genes in maize,different types materials with pyramiding high lysine(o2,o16)and waxy(wx)genes and their parents were used as test materials.The content of oil content,total starch,protein and amino acid was analyzed by using near infrared grain analyzer and fully automatic amino acid analyzer.The results showed that compared with the recurrent parent CML 530,the three-gene combination materials W 1(o2o16wx)grain oil content decreased by 20.69%,the total starch content increased by 4.22%,and the lysine content increased by 72.57%.In the four o2 and o16 two-gene aggregated materials,the total starch content of W 2(o2o16),W 3(o2o16),W 11(o2o16)and W 12(o2o16)increased by 2.92%on average;the content of lysine increased by 68.55%,67.68%,49.77%and 56.08%.Among the 7 materials aggregated by o16 and wx genes,W 4(o16wx)and W 5(o16wx)grain protein content increased by 8.10%on average;W 6(o16wx),W 7(o16wx)and W 9(o16wx)grain lysine content,respectively increased by 9.84%,12.30%and 8.66%.It could be seen that these high-quality gene polymer materials were important materials for genetic improvement of quality traits and breeding applications.