摘要
本研究选用改良M 8、N 6基本培养基和麦芽糖、蔗糖设置4种不同处理水平的诱导培养基进行花药培养。结果表明,对于大部分籼稻材料,诱导愈伤培养基添加N 6培养基和麦芽糖较为理想。以7个籼稻品种(系)为材料进行花药培养,筛选出高花药培养力基因型农香24、襄中稻3号、禾香1号和襄中稻1号,可作为单倍体育种、构建DH群体以及遗传转化的基础材料。
In this study,the improved M 8 and N 6 basic media,maltose and sucrose were used as four different treatment levels of induction medium to carry out anther culture.The results showed that callus-inducing medium added with N 6 medium and maltose were ideal for most indica rice.Seven indica rice cultivars(lines)were used for anther culture,and Nongxiang 24,Xiangzhong Dao 3,Hexiang 1 and Xiangzhong Dao 1 with high anther culture ability were screened out,which could be used as basic materials for haploid breeding,DH population construction and genetic transformation.
作者
赵沙沙
陈波
房振兵
潘高峰
潘秀才
赵红
田永宏
ZHAO Shasha;CHEN Bo;FANG Zhenbing;PAN Gaofeng;PAN Xiucai;ZHAO Hong;TIAN Yonghong(Xiangyang Academy of Agricultural Sciences,Xiangyang Hubei 441057,China)
出处
《种子》
北大核心
2023年第1期135-140,共6页
Seed
基金
湖北省农业科技创新中心重大科技研发项目(2020-620-000-002-01)
湖北省重点研发计划项目(2020BBB053)。
关键词
籼稻
花药培养
基本培养基
碳源
基因型
indica ric
anther culture
basic medium
carbon source
genotype
