基于高密度遗传图谱定位大豆蛋白质含量相关的QTL

Mapping soybean protein QTLs based on high-density genetic map

大豆是重要的粮食作物和经济作物,其籽粒蛋白约为40%,是优质植物蛋白主要来源之一。挖掘控制大豆高蛋白数量性状位点(Quantitative trait loci,QTL)以及分子标记育种对高蛋白大豆培育具有重要的意义。本研究利用蛋白含量存在明显差异的中黄35(Zhonghuang 35,ZH35)和中黄13(Zhonghuang 13,ZH13)杂交构建的包含192个株系的重组自交系群体为供试材料,通过对两亲本及RIL群体重测序,构建了包含4879个bin标记的高密度遗传图谱,总遗传距离为3760.71 cM,相邻标记间的遗传距离为0.77 cM。RIL群体及亲本分别于北京顺义和河南濮阳种植,2个环境共检测到15个蛋白含量相关QTL位点,分布于5号、12号、15号、17号、18号、19号和20号染色体,贡献率为4.36%~11.39%。其中,北京顺义和河南濮阳检测到qPro-20-1和qPro-20-3,2个QTL贡献率分别为7.65%和7.58%,重叠区域包括33个基因。本研究有助于精细定位和图位克隆大豆蛋白含量相关基因,并为进一步培育高蛋白大豆品种提供基因资源。

Soybean is an important food crop and economic crop,and its grain protein is about 40%,which is one of the main sources of high-quality vegetable protein.Mining the quantitative trait loci(QTL)that control soybean high protein and molecular marker breeding are of great significance for the breeding of high protein soybean.In this study,a recombinant inbred line population consisting of 192 lines constructed by crossing Zhonghuang 35(ZH35)and Zhonghuang 13(ZH13)with significant differences in protein content was used as the experimental materials.A high-density genetic map containing 4879 bin markers was constructed with the total genetic distance of 3760.71 cM and the genetic distance between adjacent markers of 0.77 cM by resequencing the two parents and the RIL population.The RIL population and its parents were grown in Shunyi,Beijing and Puyang,Henan,respectively.A total of 15 protein content-related QTL loci were detected in the two environments,which were distributed on chromosomes 5,12,15,17,18,19,and 20,respectively.The contribution rate was 4.36%-11.39%,among which,qPro-20-1 and qPro-20-3 were detected in Shunyi,Beijing and Puyang,Henan,respectively.The contribution rates of the two QTLs were 7.65%and 7.58%,respectively,and the overlapping regions included 33 genes.This study is helpful for fine mapping and map-based cloning of soybean protein content-related genes,and provides genetic resources for further breeding of high-protein soybean varieties.