甘薯PHB基因家族的全基因组鉴定和表达分析

Genome-wide identification and expression analysis of PHB gene family in sweet potato

蛋白质抑制素(prohibitin,PHB)是在原核生物到真核生物中发现的含有SPFH结构域的蛋白质。植物PHB基因家族参与多种不同生物过程的重要功能,包括生长发育以及对生物和非生物胁迫的响应。目前PHB蛋白在拟南芥、水稻、玉米、大豆、番茄和陆地棉等多种植物中被鉴定。但对甘薯中PHB家族的系统分析仍未确定。本研究鉴定出甘薯11个PHB基因,且对这些保守的蛋白质基序和基因结构的分析显示它们在系统发育亚群中具有高度的保守性。此外,启动子区域预测出与多种激素调节及胁迫相关的顺式作用元件,同时研究发现IbPHB基因在植物不同部位及受到不同的非生物胁迫时的表达模式存在差异。本研究系统分析了甘薯中IbPHB基因的一般特性,为甘薯及其他植物中PHB基因的功能特性研究提供了理论基础。

The prohibitins(PHB)are SPFH domain-containing proteins found in the prokaryotes to eukaryotes.Plant PHB gene family is involved in many important functions in a variety of different biological processes,including growth and development and responses to biotic and abiotic stresses.At present,PHB proteins have been identified in Arabidopsis,rice,maize,soybean,tomato,upland cotton,and other plants.However,the systematic analysis of PHB family in sweet potato is still uncertain.In this study,11 PHB genes in sweet potato were identified,and the analysis of these conserved protein motifs and gene structures showed that they were highly conserved in phylogenetic subgroups.In addition,the cis-acting elements related to various hormone regulation and stress were predicted in the promoter region and the relative expression patterns of IbPHB genes were different in different parts of plants and under different abiotic stresses.This study systematically analyzed the general characteristics of IbPHB gene in sweet potato,and provided a theoretical basis for the study of functional characteristics of PHB gene in sweet potato and other plants.