香榧精油/β–环糊精包合物的制备、表征及特性

Preparation,Characterization and Properties of Torreya Grandis Essential Oil/β-cycldextrin Inclusion Complex

目的为了解决香榧精油易挥发的问题,制备以β–环糊精为壁材、香榧精油为芯材的包合物。方法通过单因素和正交试验研究包合物的最佳制备条件,表征包合物的形态结构和香榧精油的缓释、抗菌性能。结果使用气相色谱–质谱联用(GC–MS)测试发现,香榧精油包含以柠檬烯(质量分数为27.82%)和α–蒎烯(质量分数为27.77%)为主的43种化学成分。包合物的最优制备条件为壁芯比值12、包埋温度40℃、包埋时间2 h。此条件下制备的包合物中香榧精油的包埋率为58.76%,粒径范围为0.4~1.0μm。包合物对大肠杆菌和金黄色葡萄球菌均有明显的抑菌活性。在20℃条件下,香榧精油在4 d内完全挥发,而香榧精油/β–环糊精包合物放置20 d后的精油保留率为73.66%。结论扫描电镜(SEM)、傅里叶红外光谱(FTIR)和热失重(TG)测试表明,通过分子包埋法成功制备了香榧精油/β–环糊精包合物,达到了香榧精油缓慢释放的效果。

The work aims to reduce the volatilization of Torreya grandis essential oil,and prepare inclusion complex with Torreya grandis essential oil as the core material andβ-cycldextrin as the wall material.The best preparing conditions were analyzed by single factor and orthogonal tests,and the structure of the inclusion complex and the release and the antibacterial property of Torreya grandis essential oil were studied.The gas chromatography-mass spectrometry(GC-MS)result showed that the Torreya grandis essential oil contained 43 ingredients,and the main components were limonene(27.82 wt.%)andα-pinene(27.77 wt.%).Then,the optimal preparation conditions of the inclusion complex were as follows:the wall-to-core ratio was 12,the embedding temperature was 40℃,and the embedding time was 2 h.Under these conditions,the entrapment rate of the Torreya grandis essential oil was 58.76%and the particle size of the microcapsule was 0.4-1.0μm.The inclusion complex showed antibacterial activity against Escherichia coli and Staphy-lococcus aureus.At the temperature of 20℃,the Torreya grandis essential oil volatilized completely in 4 days,but the retention rate of the Torreya grandis essential oil in the complex was 73.66%after 20 days.Scanning electron microscopy(SEM),Fourier transform infrared spectroscopy(FTIR)and thermogravimetric(TG)result show that the inclusion complex of Torreya grandis essential oil/β-cyclodextrin was successfully prepared by molecular embedding method,achieving the effect of slow release of Torreya grandis essential oil.