小长蝽线粒体基因组全序列测定与长蝽总科系统发育分析

Sequencing the complete mitochondrial genome of Nysius ericae (Schilling )(Hemiptera ∶ Lygaeidae)and phylogenetic analysis of Lygaeoidea

为了解小长蝽Nysius ericae(Schilling)线粒体基因组结构及长蝽总科的分子系统发育关系。本试验采用Illumina MiSeq测序平台对小长蝽线粒体基因进行测序,对基因组序列进行拼装、注释和特征分析,利用最大似然法和贝叶斯法构建基于12种长蝽总科昆虫线粒体全基因组核苷酸序列的系统发育树。小长蝽线粒体基因组全长为16 330 bp(GenBank登录号:MW465654),基因组包括13个蛋白编码基因(PCGs),22个tRNA基因,2个rRNA基因和1段非编码控制区。11个蛋白质编码基因的起始密码子为典型的ATN;cox1,nad4l的起始密码子为TTG。cob的终止密码子为TAG,其余蛋白编码基因的终止密码子为TAA。只有trnS1缺少DHU臂,其余tRNA基因均能形成典型的三叶草结构。12种长蝽总科昆虫线粒体全基因组序列构建的昆虫系统发育树结果显示,小长蝽与Nysius plebeius具有更近的亲缘关系,且与传统形态学分类基本一致。小长蝽线粒体基因组符合长蝽总科线粒体基因组的一般特征。结果表明小长蝽与N.plebeius的亲缘关系更近。

In order to understand the mitochondrial genome structure of Nysius ericae(Schilling)and phylogenetic analysis of Lygaeoidea.Using Illumina MiSeq sequencing platform,the mitochondrial genome of N.ericae was sequenced,the genome sequences were assembled,annotated and characterized.The phylogenetic relationship was constructed by using Maximum likelihood method and Bayesian method based on the nucleotide sequences of the whole mitochondrial genome of 12 species of Lygaeoidea.The complete mitochondrial genome of N.ericae(GenBank No.:MW465654)was 16330 bp in length,including 13 protein-coding genes(PCGs),22 tRNA genes,2 ribosomal RNA genes,and one control region.Eleven PCGs contained the typical insect start codon ATN,while cox1 and nad4l h ad TTG as the start codon.The complete stop codons for cob genes were TAG,the other PCGs had TAA as the stop codon.Only trnS1 lacked the DHU arm,and other tRNA genes all had a typical clover-leaf structure.The results of an insect phylogenetic tree constructed based on the whole mitochondrial genome sequence of 12 species of Lygaeoidea insects showed that the N.ericae was clustered with the N.plebeius,indicating that the relationship between N.ericae and N.plebeius was close,and this was basically consistent with traditional morphological classification.The mitochondrial genome of Nysius ericae(Schilling)conforms to the general characteristics of Lygaeoidea mitochondrial genome.The results showed that the phylogenetic relationship between the Nysius ericae and N.plebeius was closer.