便塞通合剂对慢传输型便秘大鼠结肠SCF/c-Kit信号通路的影响

Biansetong Mixture on colonic SCF/c-Kit signaling pathway rats with slow transit constipation

目的:探讨便塞通合剂(BST)对慢传输型便秘(STC)大鼠结肠干细胞因子(SCF)/c-Kit信号通路的影响。方法:将40只健康SD雄性大鼠分为正常组(NC组,n=10)和造模组(n=30);运用洛哌丁胺复制STC大鼠模型,造模成功后,将造模组大鼠随机分为模型组(MC组)、莫沙比利组(PC组)、便塞通合剂组(BST组),每组各10只。药物干预14 d后,比较各组大鼠一般情况、24 h粪便颗粒数、粪便含水量及肠道炭墨推进率;免疫组化和RT-qPCR检测SCF、c-Kit的表达。结果:与模型组比,BST组大鼠24 h粪便颗粒数、粪便含水量、肠道炭墨推进率均增高(P<0.05);与空白组比,模型组大鼠结肠组织c-Kit、SCF蛋白及mRNA表达水平均降低(P<0.05);与模型组比,BST组大鼠结肠组织c-Kit、SCF蛋白及mRNA表达水平均增高(P<0.05)。结论:BST可有效治疗大鼠慢传输便秘,机制可能与上调肠道SCF及c-Kit表达,加快肠道蠕动有关。

Objective:To investigate the effect of Biansetong Mixture(BST)on the SCF/c-Kit signal pathway in colon of rats with slow transit constipation(STC).Methods:40 healthy male Sprague Dawley(SD)rats were divided into two groups:10 in normal control(NC)group and 30 in model group.The STC rat model was replicated with loperamide.After successful modeling,30 rats in the model group were randomly divided into three groups:model control group(MC group),mosapride group(PC group),and Biansetong Mixture group(BST group),10 rats in each group.After 14 days of drug intervention,the general situation of rats,grain number of stool,water content of stool and small intestine promoting rate of carbon ink for 24 hours in each group were compared,and the expression of SCF and c-Kit was detected by Immunohistochemistry and RT-qPCR.Results:Compared with the MC group,the grain number of stool and the water content of stool,intestinal prompting rate of carbon ink at 24 h of rats in the BST group increased(P<0.05).Compared with the NC group,protein expression and the mRNA expression levels of SCF and c-Kit decreased in the colon tissues of rats in the MC group(P<0.05).Compared with the MC group,protein expression and the mRNA expression levels of SCF and c-Kit increased in the colon tissues of rats in the BST group(P<0.05).Conclusion:The mechanism of BST in treating STC may be through increasing the expression of SCF and c-Kit in the intestine,speeding up the intestinal peristalsis,to relieve and treat constipation.