可植入无线Micro LED光遗传学硬件设计

Design of implantable wireless Micro LED optogenetic hardware

为将光遗传技术用于自由移动生物体的体内视觉神经调控,文中提出一种新的无线能量传输、可植入、光源参数可调、多通道的光遗传硬件结构。该硬件结构主要包括Micro LED阵列多通道光源、nRF52832控制器、光驱动电路、无线能量传输电路,硬件整体尺寸为18 mm×18 mm×2 mm,质量为1.54 g,满足轻量化植入的要求。其次,设计并流片Micro LED光源阵列,单颗光源的尺寸为60μm×60μm;并在此基础上为每颗光源设计光导,实现光导直径在10μm左右,能够完成神经元级的光刺激。最后,将光源阵列的参数与光源阵列组合,运用深度学习的方法进行优化,实现光源的最优刺激。实验结果表明,光源满足光遗传的要求。

In order to apply the optical genetic technology to the visual nerve regulation of free⁃moving organisms in vivo,a new wireless energy transmission,implantable,light source parameter adjustable,multi⁃channel optical genetic hardware structure is proposed.The hardware structure mainly includes Micro LED array multi⁃channel light source,nRF52832 controller,optical drive circuit and wireless energy transmission circuit.Its overall volume is 18 mm×18 mm×2 mm,and its weight is 1.54 g,which can meet the requirements of lightweight implantation.The design and tape⁃out for the Micro LED light source array are conducted.The size of a single light source is 60μm×60μm.On this basis,the optical guide is designed for each light source to achieve the optical guide diameter of about 10μm,so as to complete the light stimulationin neuronlevel.The light source array parameters are combined with the light source array,and their combination is optimized by means of the deep learning method to achieve the optimal stimulation of the light sources.The experimental results show that the light source can meet the optogenetic requirements.