光生物调节促进脐带间充质干细胞移植后脊髓损伤大鼠运动功能恢复的实验研究

Effects of photobiomodulation combined umbilical cord mesenchymal stem cell transplantation on motor function recovery of spinal cord injury rats

目的:探讨光生物调节(PBM)联合脐带间充质干细胞移植对脊髓损伤大鼠运动功能恢复的作用。方法:以1.5、3、6、12 J/cm^(2)的能量密度对间充质干细胞进行激光照射,在第3天用噻唑蓝(MTT)法确定增殖率最佳的能量密度。细胞移植前,用最佳的能量密度进行激光照射,将32只雄性SD大鼠随机分为脊髓损伤组、干细胞移植组、激光照射组和联合治疗组,每组8只。分别在造模后1、3、7、14、21 d进行BBB评分和斜板实验;在造模后21 d进行苏木精-伊红(HE)染色和尼氏(Nissl)染色。结果:能量密度为12 J/cm^(2)的激光照射能够加速细胞增殖(P<0.05)。造模后,联合治疗组的BBB评分高于其他组(均P<0.05),运动功能恢复明显。斜板实验中,联合治疗组和激光照射组的表现也优于其他组。苏木精-伊红染色结果表明,联合治疗组空洞面积明显减少,炎性反应最轻;尼氏小体染色变深,脊髓损伤明显降低。结论:PBM能够促进脐带间充质干细胞移植后脊髓损伤大鼠运动功能恢复,对大鼠脊髓损伤有明显的治疗作用。本研究结论为脊髓损伤的治疗提供依据。

Objective To explore the effect of photobiomodulation(PBM)combined with umbilical cord mesenchymal stem cell transplantation on motor function recovery in rats with spinal cord injury.Methods The mesenchymal stem cells were irradiated with a laser energy density of 1.5,3,6 and 12 J/cm^(2).The optimal energy density was screened by the MTT method on the 3rd day.Before cell transplantation,32 male SD rats were randomly divided into the spinal cord injury group,which was injected with normal saline without cells;the stem cell transplantation group,which was injected with stem cells in the injury model;the laser irradiation group,which was injected with cell-free saline and laser irradiation;and the combined treatment group,which was treated with cell transplantation and laser irradiation.BBB score and inclined plate test were performed on the 1st,3rd,7th,14th and 21st day,and hematoxylin-eosin staining and Nissl staining were performed on the 21st day.Results The laser irradiation with an energy density of 12 J/cm^(2) can accelerate cell proliferation(P<0.05).After the modeling,the BBB score of the combined treatment group was higher than that of the other groups(all P<0.05),and the motor function recovered significantly.In the inclined plate experiment,the performance of the combined treatment group and the laser irradiation group was also better than that of other groups.The results of hematoxylin-eosin staining showed that the cavity area in the combined treatment group was significantly reduced,and the inflammatory reaction was the lightest.The staining of Nissl bodies became deeper,and the spinal cord injury was significantly reduced.Conclusions PBM can promote the recovery of motor function in rats with spinal cord injury after the transplantation of umbilical cord mesenchymal stem cells,and has an obvious therapeutic effect on spinal cord injury in rats.This study provides a basis for the treatment of spinal cord injury.