基于数据挖掘和网络药理学探讨宋爱莉教授治疗肉芽肿性小叶性乳腺炎的经验

Experience of professor Song Aili in the treatment of granulomatous lobular mastitis based on data mining and network pharmacology

目的 基于数据挖掘及网络药理学方法探讨宋爱莉教授治疗肉芽肿性小叶性乳腺炎(GLM)的用药规律及核心药对作用机制。方法 收集2021年6月—2022年6月山东省中医药大学附属医院宋爱莉教授门诊及住院诊治的GLM患者服用的中药处方。通过中医传承辅助平台挖掘相关用药规律,并利用网络药理学分析核心药对的作用机制。结果 共有248首中药处方纳入本研究,金银花-连翘药对为宋爱莉教授治疗本病的核心药对;金银花-连翘药对的重要活性成分有槲皮素、木犀草素、汉黄芩素等;金银花-连翘药对作用于GLM的关键靶点有肿瘤坏死因子(TNF)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、CXC趋化因子配体8(CXCL8)、趋化因子配体2(CCL2)等;药对活性成分调控的主要信号通路有白细胞介素-17(IL-17)信号通路、Toll样受体信号通路、TNF信号通路等。结论 宋爱莉教授辨证多从阳证论治GLM,以化痰散瘀、透脓解毒、益气养血为核心治法,金银花-连翘为核心药对,其可能通过调控IL-17信号通路、Toll样受体信号通路、TNF信号通路发挥治疗GLM的效果。

Objective To explore the medication rules of professor Song Aili in treating granulomatous lobular mastitis(GLM)and the mechanism of core herbal pairs based on data mining and network pharmacology methods.Methods From June 2021 to June 2022,the traditional Chinese medicine prescriptions taken by GLM patients treated by Professor Song Aili in the outpatient and inpatient departments of the Affiliated Hospital of Shandong University of Traditional Chinese Medicine were collected.Relevant medication rules were explored through the TCM inheritance auxiliary platform,and the mechanism of core herbal pairs was analyzed by network pharmacology.Results A total of 248 traditional Chinese medicine prescriptions were included in this study,and the herbal pair of Jinyinhua(lonicerae japonicae flos)with Lianqiao(forsythiae fructus)was the core herbal pair used by professor Song Aili for treatment of this disease;the important active ingredients of the herbal pair of Jinyinhua with Lianqiao were quercetin,luteolin and wogonin;the key targets of the herbal pair of Jinyinhua with Lianqiao on GLM were tumor necrosis factor(TNF),interleukin-6(IL-6),interleukin-1β(IL-1β),CXC chemokine ligand 8(CXCL8),chemokine ligand 2(CCL2)and etc.;the main signal pathways regulated by active ingredients of herbal pair included interleukin-17(IL-17)signaling pathway,Toll-like receptor signaling pathway,TNF signaling pathway and etc.Conclusion Professor Song Aili mainly treat GLM from Yang syndrome based on syndrome differentiation.Removal of phlegm and blood stasis,permeation of sepsis and detoxification,and nourishment of qi and blood are regarded as the core therapeutic method,and Jinyinhua with Lianqiao as core herbal pair that may obtain therapeutic effect on GLM by regulating IL-17 signaling pathway,Toll-like receptor signaling pathway and TNF signaling pathway.