摘要
萝卜根肿病病理研究十分有限。本研究使用芸薹根肿菌对萝卜幼苗根部进行侵染实验,观察侵染规律,并基于LC-MS/MS的代谢组检测技术对感根肿病和健康萝卜根部进行代谢物检测。结果表明根肿菌可于接种后72 h内完成侧根侵染,但病症主要出现于中柱分裂期,推测在中柱分裂期时根肿菌初步进入孢子增殖阶段。感病萝卜在生长后期根部生物量下降,根肿部位维管组织消失、细胞被根肿菌填充。对中柱分裂期萝卜进行代谢组检测,结果表明对照与感病样品之间分离明显;共检测到差异物6 354个,主要包括花生四烯酸、萜类、淀粉等。
The pathological research on radish clubroot is very preliminary. In this study, we used Plasmodiophora brassicae to infect the roots of radish seedlings to observe the infection pattern, and the metabonomic detection technique based on LC-MS/MS was used to detect metabolites in the roots of infectious diseases and healthy radish. The results showed that P. brassicae completed lateral root infection within 72 h after inoculation, symptoms mainly appeared in the cortex splitting stage of radish. It is speculated that P. brassicae initially arrivals the spore proliferation stage during the division of the cortex cells. The root biomass of susceptible radish decreased significantly in the later stage of growth, the vascular tissue in the diseased root disappeared, and the cells were filled by spores. The metabonomic detection of radish at cortex splitting stage showed there was a significant separation between control and susceptible samples;a total of 6 354 different expressed metabolites were detected, mainly including arachidonic acid, terpenoids, starch and other substances.
作者
陆锦彪
裴芸
祖贵东
毛妃凤
刘军林
张万萍
李经纬
LU Jinbiao;PEI Yun;ZU Guidong;MAO Feifeng;LIU Junlin;ZHANG Wanping;LI Jingwei(College of Agriculture,Guizhou University,Guiyang 550025,China;Vegetable Research Institute,Guizhou University,Guiyang 550025,China;Weining County Bureau of Agriculture and Rural Affairs,Bije,Guizhou 551700,China;Guizhou Province Fruit Tree and Vegetable Workstation,Guiyang 550001,China)
出处
《植物生理学报》
CAS
CSCD
北大核心
2023年第1期101-112,共12页
Plant Physiology Journal
基金
贵州省科技计划项目基金(黔科合基础-ZK[2021]一般153)
贵州大学培育项目基金(贵大培育[2019]41号)
贵州大学引进人才科研项目[贵大人基合字(2019)号]。
关键词
萝卜
根肿病
侵染
代谢组
radish
clubroot
infection
metabolome