盾叶秋海棠叶片高频再生体系的建立

Establishment of high-frequency regeneration system of Begonia peltatifolia from leaves

盾叶秋海棠(Begonia peltatifolia)利用叶片扦插繁殖困难,插穗很少能再生不定芽。为了更好地保护和利用该种资源,以其叶片作为外植体建立高效的组培体系。结果表明:最适用于盾叶秋海棠叶片愈伤组织和不定芽诱导的培养基均为MS+6-BA 1.00 mg·L^(-1)+NAA 0.15 mg·L^(-1),诱导率分别高达97.67%和71.00%;不定芽增殖最适培养基为MS+6-BA 1.25 mg·L^(-1)+NAA 0.05 mg·L^(-1),增殖系数达20.10;不定芽生根最适培养基为1/2MS+NAA 0.50 mg·L^(-1)(单芽)和1/2MS+NAA 0.10 mg·L^(-1)(丛芽),生根率分别为91.11%和100.00%。丛芽芽色深绿、根系健壮,移栽成活率达到90.63%以上。

It is difficult to propagate Begonia peltatifolia by leaf cuttings which rarely regenerate shoots. For better protection and utilization of this species, its leaves are used as explants for efficient in vitro propagation. The results indicated that the optimum callus and shoots induction medium for the leaves were MS+6-BA 1.00 mg·L^(-1)+NAA 0.15 mg·L^(-1), with 97.67% and 71.00% induction rate. The proliferation coefficient of clustered shoots reached 20.10 in the MS+6-BA 1.25 mg·L^(-1)+NAA 0.05 mg·L^(-1)as optimum medium. The optimum rooting medium for clustered shoots was 1/2MS+NAA 0.50 mg·L^(-1)(single shoot) and 1/2MS+NAA 0.10 mg·L^(-1)(cluster shoots). The rooting rate of them was 91.11% and 100.00%, respectively.The shoots in the shoot-cluster state were dark green, and the roots were robust. After being transplanted,about 90.63% of plantlets survived.