摘要
从焦化厂附近农田污染土壤及污水处理厂的活性污泥中驯化筛选到4个菲、萘复合降解菌群,其中驯化自活性污泥的复合菌群96 h内对菲、萘的降解效率分别达到85.90%和79.45%,驯化自污染土壤的复合菌群对菲和萘降解效率分别为52.63%和52.15%。为比较不同复合降解菌群的物种结构及多样性,进一步采用Illumina Miseq高通量测序技术测定16S rDNA的V3~V4区序列。4个复合菌群共产生420 771条优化序列,聚为10 696个操作分类单元(OTU),且各菌群中绝大多数OTU是其独有的。不同菌群的物种丰度大小排序为:污染土壤来源的菲降解菌群>活性污泥来源的萘降解菌群>污染土壤来源的萘降解菌群>活性污泥来源的菲降解菌群,物种多样性大小排序为:污染土壤来源的菲降解菌群>活性污泥来源的萘降解菌群>活性污泥来源的菲降解菌群>土壤来源的萘降解菌群。从属分类水平看,污染土壤来源的菲降解菌群中优势菌有肠杆菌属(Enterobacter)(38.21%)、丛毛单胞菌属(Comamonas)(32.78%)和不动杆菌属(Acinetobacter)(20.49%),萘降解菌群中有肠杆菌属(85.16%)和不动杆菌属(9.67%);活性污泥来源的菲降解菌群中优势菌为寡养食单胞菌属(Stenotrophomonas)(90.58%),萘降解菌群中为不动杆菌属(52.74%)和肠杆菌属(40.11%)。本研究证明驯化自焦化厂附近农田污染土壤以及活性污泥的菲、萘降解菌群的物种丰度及多样性均非常高,是用于治理多环芳烃污染土壤或水体的重要资源。
In this paper, four compound bacteria for degrading phenanthrene or naphthalene were acclimated from the polluted farmland soil nearby a coke-plant as well as the activated sludge from a waste water plant, in which the compound bacteria from the activated sludge gave a degradation efficiency of 85.90% and 79.45% in 96 hours to phenanthrene and naphthalene, respectively and those from the polluted farmland soil with a degradation efficiency of 52.63% and 52.15% to phenanthrene and naphthalene, respectively. In order to compare the community structure and diversity in these compound bacteria, the V3-V4 regions in bacterial 16S rDNA were sequenced using the Illumina Miseq platform, by which a total of 420,771 filtered sequences of 16S rDNA were obtained from 4 compound bacteria and subsequently clustered into 10,696 OTUs(operational taxonomic units), with most of OTUs unique for each community. The order of species richness for different community was the soil phenanthrene-degrading compound bacterium>the sludge naphthalene-degrading compound bacterium>the soil naphthalene-degrading compound bacterium>the sludge phenanthrene-degrading compound bacterium, while that of species diversity was the soil phenanthrene-degrading compound bacterium>the sludge naphthalene-degrading compound bacterium>the sludge phenanthrene-degrading compound bacterium > the soil naphthalene-degrading compound bacterium. On the genus-level classification, the dominant bacteria in the soil phenanthrene-degrading compound bacterium included Enterobacter(38.21%), Comamonas(32.78%) and Acinetobacter(20.49%), and those in the soil naphthalenedegrading compound bacterium were comprised of Enterobacter(85.16%) and Acinetobacter(9.67%). On the other hand, the dominant bacteria in the sludge phenanthrene-degrading compound bacterium came from Stenotrophomonas(90.58%), and those for the sludge naphthalene-degrading compound bacterium consisted of Acinetobacter(52.74%) and Enterobacter(40.11%). This study developed the phenanthrene-or naphthalene-degrading compound bacteria with high species richness and diversity, which can be used to remediate the polluted soil or water by polycyclic aromatic hydrocarbons.
作者
汪从胜
高璐
成亮
李霞
蒋建雄
WANG Congsheng;GAO Lu;CHENG Liang;LI Xia;JIANG Jianxiong(School of Environment and safety Engineering,Jiangsu University,Zhenjiang 212013,China)
出处
《东北农业科学》
2023年第1期108-115,共8页
Journal of Northeast Agricultural Sciences
基金
国家自然科学基金项目(31671754)
江苏大学高级人才基金项目(15JDG012)
镇江市重点研发计划项目(SH2022011)。