谷子MADS-box基因家族的鉴定和表达分析

Genome-wide identification and expression analysis of MADS-box genes in foxtail millet

MADS-box基因是真核生物中一类重要的转录因子,参与调控多项植物的生长发育过程。然而关于谷子穗发育的MADS-box基因研究比较少。本研究使用序列相似性检索,在Phytozome 13.0数据库中筛选并且鉴定出了68个谷子MADS家族成员,并对这些家族成员的物理化学性质、系统发育树、染色体定位、表达谱等进行了全面的分析。结果表明,谷子MADS家族成员在染色体上分布不均匀,可以分为5个亚族。通过组织特异性表达谱分析得到,多数MADS基因在穗中表达量要高于其他器官。此外利用转录组测序技术对发育初期的谷穗和成熟期的谷穗进行了转录组测序分析,筛选到数个与谷穗分生组织发育相关MADS-box基因。为进一步揭示MADS-box基因在谷子穗发育过程中的作用奠定了重要的基础。

MADS-box gene is an important transcription factor in eukaryote,which is involved in regulating a number of plant growth and development processes.While there are few studies on MADS-box genes of foxtail millet during panicle development.In this study,a genome-wide searching of foxtail millet MADS-box genes was performed in Phytozome 13.0 using sequence similarity search,and 68 foxtail millet MADS-box genes were screened and identified.The physical chemical property,phylogenetic tree,chromosome location,and expression profile of these family members were analyzed.Results showed that the members of the SiMADS family were divided into five subfamilies and unevenly distributed on chromosomes.According to tissue-specific expression patterns,the expression of most SiMADS genes in spike was higher than that in other tissues.Additionally,transcriptome analysis of spike in early stage and mature stage was conducted,and several spike differentiation related SiMADS genes were detected.The study provides important foundation for further revealing the function of MADS-box genes during foxtail millet inflorescence development.