CYP2R1基因多态性与维生素D水平和肺结核病易感性的相关性研究

Correlation among CYP2R1 gene polymorphisms,vitamin D level and susceptibility to pulmonary tuberculosis

目的探讨CYP2R1基因rs10741657、rs10766197、rs1993116位点的多态性与维生素D水平和结核病之间的关系。方法选取青海省某医院174例结核病患者为病例组,202例同期体检健康者为对照组,通过聚合酶链反应(PCR)技术扩增rs10741657、rs10766197、rs1993116位点并测定基因型,酶联免疫吸附试验(ELISA)测定维生素D浓度。结果rs10741657基因多态性与结核病相关(χ^(2)=6.884,P=0.032),未发现rs10766197、rs1993116多态性与结核病相关。结核病患者中,rs10741657位点GG基因型携带者体内维生素D水平低于AA基因型(P=0.033)和AG基因型(P=0.034),未发现rs10766197、rs1993116位点基因型与维生素D水平相关。结论rs10741657基因多态性与维生素D水平和结核病的发病相关。

Objective To explore the relationship among the polymorphisms of CYP2R1 gene at rs10741657,rs10766197,and rs1993116 loci,vitamin D levels and susceptibility to tuberculosis.Methods 174 tuberculosis patients from a hospital in Qinghai Province were selected as the case group and 202 healthy individuals underwent physical examination during the same period were selected as the control group.Polymerase chain reaction(PCR)was used to amplify the rs10741657,rs10766197 and rs1993116 loci,and determine the genotypes.The vitamin D level was determined by enzyme-linked immunosorbent assay(ELISA).Results rs10741657 gene polymorphism was associated with tuberculosis(χ^(2)=6.884,P=0.032),while no association was found between rs10766197,rs1993116 polymorphisms and tuberculosis.Among tuberculosis patients,individuals carrying the GG genotype at the rs10741657 locus had lower vitamin D level than those with the AA genotype(P=0.033)and AG genotype(P=0.034).The genotypes at the rs10766197 and rs1993116 loci were not found to be associated with vitamin D levels.Conclusion Gene polymorphism at the rs10741657 locus is associated with vitamin D levels and the development of tuberculosis.