烟草连作病害土壤与连作非病害土壤的细菌群落差异性比较

Comparison on differences of bacteria community between tobacco continuous cropping diseased soil and continuous cropping non-diseased soil

通过对烟草连作病害土壤、连作非病害土壤及新垦植烟土壤细菌群落结构间的差异性比较,为以微生物手段减少烟草连作病害提供一定的理论依据。采用宏基因组高通量测序技术,测定分析三类土壤的细菌群落结构。结果表明,连作病害土壤细菌总OTUs与独有OTUs最多,群落结构相似,同源性高;连作病害土壤中的蓝细菌门(Cyanobacteria)、拟杆菌门(Bacteroidetes)、假单胞菌属(Pseudomonas)、铁矿沙单孢菌属(Arenimonas)、厌氧绳菌属(Bellilinea)、节杆菌属(Arthrobacter)、黄杆菌属(Flavobacterium)、类诺卡氏菌属(Nocardioides)占比显著高于连作非病害土壤和新垦土壤。连作非病害土壤样本间细菌群落结构差异较大,同源性较低。新垦土壤细菌慢生根瘤菌属(Bradyrhizobium)、酸土单胞菌属(Aciditerrimonas)、分枝杆菌属(Mycobacterium)、鞘氨醇单胞菌属(Sphingomonas)、红游动菌属(Rhodoplanes)5个属所占比例较高,但硝化螺旋菌属(Nitrospira)占比较低,细菌群落结构各具自身特点。

The differences of bacteria community structure among tobacco continuous cropping diseased soil,continuous cropping non-diseased soil and newly cultivated soil were compared,so as to provide a theoretical basis for reducing tobacco continuous crop⁃ping diseases through microbial means.Metagenome high-throughput sequencing technology was used to compare bacterial communi⁃ties of three types of soils.The results showed that,the number of total and unique OTUs of bacteria in continuous cropping diseased soil was the highest,and the bacterial community was very similar and had high homology.Proportions of Cyanobacteria,Bacteroides,Pseudomonas,Arenimonas,Bellilinea,Arthrobacter,Flavobacterium and Nocardioids were significantly higher in continuous cropping diseased soil than those in continuous cropping non-diseased soil and newly cultivated soil.The bacterial community structure of con⁃tinuous cropping non-diseased soil was quite different,and the homology was low.Bradyrhizobium,Aciditerrimonas,Mycobacterium,Sphingomonas,and Rhodoplanes had relatively high proportions,but the proportion of Nitrospira was low in newly cultivated soil,and the bacterial community structure had its own characteristics.