下调RNA甲基化酶NSUN2表达对脑胶质瘤U87细胞增殖、侵袭、迁移的影响

Effects of down-regulated expression of RNA methylase NSUN2 on proliferation,invasion,and migration of glioma U87 cells

目的探讨下调NOP2/Sun RNA甲基转移酶家族成员2(NSUN2)表达对脑胶质瘤细胞增殖、侵袭、迁移的影响。方法体外培养正常胶质细胞HEB、胶质瘤细胞系(A172、U251、U87),免疫印迹法检测NSUN2蛋白表达水平;用shNSUN2慢病毒(sh-NSUN2组)及shRNA scramble慢病毒(sh-CON组)感染U87细胞下调NSUN2表达,用CCK-8法检测细胞增殖活力,Transwell实验检测细胞侵袭和迁移。结果与正常胶质细胞HEB相比,胶质瘤细胞系A172、U251、U87的NSUN2蛋白表达量均明显增高(P<0.05)。与sh-CON组比较,sh-NSUN2组NSUN2蛋白表达水平、细胞增殖活力、侵袭能力和迁移能力均明显降低(P<0.05)。结论胶质瘤NSUN2呈高表达,下调其表达明显抑制胶质瘤细胞增殖、侵袭和迁移。

Objective To investigate the effect of down-regulating the expression of NOP2/Sun RNA methyltransferase family member 2(NSUN2)on proliferation,invasion,and migration of glioma cells.Methods HEB and glioma cell lines(A172,U251,U87)were cultured in vitro,and NSUN2 protein expression levels were detected using the western blotting.U87 cells were infected with shNSUN2 lentivirus(sh-NSUN2 group)and shRNA scramble lentivirus(sh-CON group)to dowm-regulate NSUN2 expression.Cell proliferation activity was detected using the CCK-8 method,and cell invasion and migration were detected using the Transwell assay.Results Compared with HEB cells,expressions of NSUN2 protein in A172,U251,and U87 cells were significantly increased(P<0.05).Compared with the sh-CON group,NSUN2 protein expression level,cell proliferation activity,invasion ability,and migration ability of the sh-NSUN2 group were significantly decreased(P<0.05).Conclusions NSUN2 is highly expressed in gliomas,and down-regulation of NSUN2 can inhibit the proliferation,invasion,and migration of glioma cells.