水稻VLN基因家族的鉴定与生物信息学分析

Identification and Bioinformatics Analysis of Rice VLN Gene Family

绒毛蛋白(VLN)通过调节微丝骨架的动态变化过程参与植物的生长发育与逆境胁迫响应,因此利用生物信息学方法在全基因组水平上对水稻VLN进行鉴定与分析,结果表明:水稻基因组共筛选鉴定出5个OsVLNs,分布于3号、4号、5号、6号和8号染色体上,均为亲水性蛋白且多为稳定蛋白;进化分析表明5个成员可能来自于一个共同祖先,在进化中形成三个亚族;OsVLNs的基因结构存在差别,基序较为保守,蛋白二级结构以α-螺旋和无规则卷曲为主;基因启动子区含有与植物激素和非生物胁迫响应相关的顺式作用元件,OsVLNs参与水稻的逆境胁迫并调节其各项生命历程。本研究将为进一步分析水稻绒毛蛋白对生长发育和非生物胁迫应答调控作用提供理论指导。

Villin(VLN)participates in plant growth and development and response to stress by regulating the dynamic changes of the microfilament skeleton.In this study,bioinformatics methods were used to identify and analyze rice VLN at the genome-wide level.The results showed that 5 OsVLNs were identified in the rice genome,which were distributed on chromosomes 3,4,5,6 and 8,all of which were hydrophilic proteins and mostly stable proteins.Evolutionary analysis showed that the five members maybe have come from a common ancestor,forming three subfamilies in evolution.There are differences in the gene structure of OsVLNs,the motifs are relatively conservative,and the secondary structure of the protein is mainlyα-helix and random coil.The gene promoter region contains cis-acting elements related to plant hormones and abiotic stress responses.OsVLNs are involved in rice stress and regulate its various life processes.The study will provide theoretical guidance for further analysis of the regulation of rice villin on growth and development and abiotic stress responses.