载万古霉素聚乙二醇功能化中空介孔硅球缓释药物的制备及其抗菌活性研究

Preparation and antibacterial activity of polyethylene glycol functionalizedhollow mesoporous silica loaded vancomycin nanocomposite

目的:万古霉素是一种具有多重杀菌机制的糖肽类抗菌药物,但因其代谢快和易于产生细菌耐药性等特点,影响其抗菌作用,开发新型基于万古霉素的纳米缓释药物具有重要意义。方法:利用模板法成功制备了聚乙二醇功能化分散性好的中空介孔纳米硅球药物载体,利用红外光谱、透射电镜、动态光散射及热重分析等手段进行了表征分析。结果:该载体对万古霉素的载药量及包封率高达154.9 mg/g和77.5%,24 h万古霉素药物累积释放率为47.4%,细胞存活率均高于85.0%,抗菌活性显著增强。结论:基于中空介孔纳米硅球载体万古霉素药物在药物缓释及生物利用度提升方面具有应用价值,为新型抗菌材料的发展提供了参考。

Objective:Vancomycin is a kind of glycopeptide antibacterial drug with multiple bactericidal mechanisms.However,its rapid metabolism and susceptibility to bacterial resistance affect its antibacterial effect.Development of novel nano sustained release drug based on vancomycin will be of great significance.Methods:Polyethylene glycol functionalized hollow mesoporous silica nanoparticles(HMS-PEG)as the drug carrier with good water solubility was successfully prepared by template method and characterized by infrared spectroscopy,transmission electron microscope,dynamic light scattering and thermogravimetric analysis.Results:The drug loading and encapsulation rate of vancomycin were up to 154.9 mg/g and 77.5%,and the cumulative release rate of vancomycin in 24 hours was 47.4%.Meanwhile,the cell survival rate was higher than 85.0%and the antibacterial activity of HMS-PEG-van was significantly enhanced.Conclusion:Vancomycin based on hollow mesoporous silica spheres has potential application value in drug release and bioavailability improvement,which provides reference for the development of new antibacterial materials.