血清LDL-P与LDL-C检测结果一致性分析

Consistency of serum LDL-P and LDL-C determination results

目的探讨基于垂直密度梯度离心(VAP)法检测低密度脂蛋白颗粒(LDL-P)与生化均相法检测低密度脂蛋白胆固醇(LDL-C)的一致性。方法选取冠状动脉粥样硬化性心脏病患者417例和健康体检者154名,采用VAP法检测LDL-P,采用生化均相法检测LDL-C。分别以3.37mmol/L和1000nmol/L作为LDL-C和LDL-P参考区间的上限。将LDL-C和LDL-P结果一致的样本纳入A组,再细分为A1组(LDL-C和LDL-P结果均正常)和A2组(LDL-C和LDL-P结果均升高);将LDL-C和LDL-P结果不一致的样本纳入B组,再细分为B1(LDL-C正常、LDL-P升高)和B2组(LDL-C升高、LDL-P正常)。采用Pearson相关分析评估各项指标之间的相关性。结果Pearson相关分析结果显示,LDL-C与LDL-P呈正相关(r=0.666,P=0.000)。A组合计为181例(31.70%),其中A1组95例(16.64%)、A2组86例(15.06%);B组合计为390例(68.30%),其中B1组373例(65.32%)、B2组17例(2.98%)。结论LDL-C与LDL-P不一致的情况主要为LDL-P升高,临床应关注LDL-P检测结果,避免对LDL-C水平正常人群的漏检。

Objective To investigate the consistency between the determination of low-density lipoprotein particles(LDL-P)by vertical auto profile(VAP)and the determination of low-density lipoprotein cholesterol(LDL-C)by biochemical homogeneity.Methods A total of 417 patients with coronary atherosclerotic heart disease and 154 healthy subjects were enrolled to determine LDL-P by VAP and LDL-C level by biochemical homogeneity.The 3.37 mmol/L and 1000 nmol/L were used as the upper limits of the reference intervals for LDL-C and LDL-P,respectively.The samples with consistent LDL-C and LDL-P results were included in group A and classified into group A1(both LDL-C and LDL-P results were normal)and group A2(both LDL-C and LDL-P results were elevated)according to the determination results.The samples with inconsistent LDL-C and LDL-P results were classified into group B,and they were classified into B1(LDL-C normal,LDL-P elevated)and B2(LDL-C elevated,LDL-P normal).Pearson correlation analysis was used to assess the correlation between the indicators.Results The results of Pearson correlation analysis showed that LDL-C and LDL-P were positively correlated(r=0.666,P=0.000).There were 181 cases(31.70%)in group A,including 95 cases(16.64%)in group A1 and 86 cases(15.06%)in group A2.There were 390 cases(68.30%)in group B,including 373 cases(65.32%)in group B1 and 17 cases(2.98%)in group B2.Conclusions The inconsistency between LDL-C and LDL-P is mainly present in patients with high LDL-P,suggesting that LDL-P should be clinically concerned to prevent missed determination in subjects with normal LDL-C levels.