南方养殖鲍优选群体杂合子一代热耐受性分析

Thermotolerance of the Selected Abalone Population’s First Generation of Heterozygotes Cultured in the South of China

以同月龄的南方鲍普通养殖群体(皱纹盘鲍)种苗为对照,采用动态法和静态法研究南方养殖鲍优选群体杂合(♂亲本(西氏鲍♀×皱纹盘鲍♂)×♀亲本(绿鲍♀×皱纹盘鲍♂))子一代种苗的热耐受性,并对其肝胰腺超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)和溶菌酶(LZM)的热胁迫响应情况进行考察。实验结果表明:南方养殖鲍优选群体杂合子一代种苗的高起始致死温度为(33.36±0.03)℃,略高于对照组的(33.13±0.02)℃;当初始水温分别为21.0,24.0,27.0℃时,南方养殖鲍优选群体杂合子一代种苗的最大临界温度分别达到(30.27±0.45),(32.57±0.31),(33.07±0.25)℃,显著高于对照组的(28.80±0.36),(30.87±0.40),(31.67±0.32)℃;持续升温环境下,南方养殖鲍优选群体杂合子一代种苗的热耐受指数高达(1918.75±208.11)℃·h,远高于对照组的(922.56±138.18)℃·h;高温胁迫下,南方养殖鲍优选群体杂合子一代种苗的肝胰腺酶SOD、CAT、ACP、LZM活性分别从(9.02±0.45)U/mg提升至(21.96±2.02)U/mg、从(7.22±0.77)U/mg提升至(11.07±1.40)U/mg、从(63.38±5.48)U/mg提升至(175.12±18.30)U/mg、从(42.45±4.87)U/mg提升至(112.71±7.47)U/mg,与对照组相比较,其肝胰腺酶SOD、CAT、ACP、LZM具有更好的热胁迫响应效果和高温适应性。

Using the seedlings of Haliotis discus hannai as a control,the thermotolerance of the first generation of heterozygous seedlings(male parents(Haliotis sieboldii♀×Haliotis discus hannai♂)×female parents(Haliotis fulgens♀×Haliotis discus hannai♂))of the selected abalone population(SAPFGH)was studied by dynamic and static methods,and the activity expression of hepatopancreatic superoxide dismutase(SOD),catalase(CAT),acid phosphatase(ACP)and lysozyme(LZM)in response to thermal stress were investigated.The results showed that the upper initial lethal temperature 168 h UILT 50 of the selected abalone population’s first generation of heterozygous seedlings cultured in the south of China was(33.36±0.03)℃,which was slightly higher than(33.13±0.02)℃of the control experimental animals.When the initial water temperature was 21.0℃,24.0℃and 27.0℃,the critical temperature maximum(CTM)of the seedlings reached(30.27±0.45)℃,(32.57±0.31)℃and(33.07±0.25)℃respectively.Under the same conditions,its value was significantly higher than that of the control experimental animals at(28.80±0.36)℃,(30.87±0.40)℃and(31.67±0.32)℃.Under the condition of initial temperature rising slowly at 24.0℃,the upper thermal tolerance index(UTT)of the SAPFGH seedlings was as high as(1918.75±208.11)℃·h,which was much higher than(922.56±138.18)℃·h in the control animals.The activities of hepatopancreatic enzymes(SOD,CAT,ACP and LZM)of the SAPFGH seedlings after experiencing high temperature stress of 26.0-32.0℃for 2 hours and returning to the initial temperature environment of 24.0℃can be increased from(9.02±0.45)U/mg to(21.96±2.02)U/mg,from(7.22±0.77)U/mg to(11.07±1.40)U/mg,from(63.38±5.48)U/mg to(175.12±18.30)U/mg,and from(42.45±4.87)U/mg to(112.71±7.47)U/mg,respectively,compared with the control experimental animals,the hepatopancreatic enzymes(SOD,CAT,ACP and LZM)have better response effect to thermal stress and high temperature adaptability.