河蚬汤纳米颗粒细胞毒性及对大鼠腹腔巨噬细胞吞噬功能的影响

Cytotoxicity of Freshwater Clam Soup Incidental Nanoparticles and Effect on Phagocytosis of Murine Peritoneal Macrophages

许多传统食品中存在大量的伴生性纳米颗粒,其纳米尺度的构造赋予它们特殊的活性与功能,然而这类纳米颗粒对人体细胞的影响尚未被充分揭示。以河蚬汤及其伴生纳米颗粒为例,采用狗肾上皮细胞(MDCK)、人结肠腺癌细胞(Caco-2)、人正常肝细胞(L-02)和大鼠原代腹腔巨噬细胞模型,研究以液相色谱方法分离制备的河蚬汤纳米颗粒的细胞毒性,以及其对大鼠原代腹腔巨噬细胞功能的影响。实验结果表明:15.63~500.00μg/mL河蚬汤伴生纳米颗粒对4种细胞均无明显毒性;这些纳米颗粒能被大鼠原代腹腔巨噬细胞吞噬,对正常巨噬细胞的膜电位和吞噬功能无明显影响,抑制AAPH诱导氧化应激引起的细胞膜超极化,拮抗线粒体的氧化应激与细胞吞噬功能损伤。抗氧化测试结果显示,河蚬汤伴生纳米颗粒具有FRAP、ABTS抗氧化活力,但不具有ORAC活性。研究旨在为后续深入开展河蚬汤及其伴生纳米颗粒的功效以及作用机制提供必要的基础数据和理论支持。

There are a large number of incidental nanoparticles endowed with special activities and functions by their nano-scale structures in some traditional food systems,but the effects of the incidental nanoparticles on the human cells remain unclear.In the case study of freshwater clam soup and its incidental nanoparticles prepared by liquid chromatography,the cytotoxicity effects of freshwater clam soup and its nanoparticles on MDCK,Caco-2,L-02,and murine peritoneal macrophages were evaluated.Besides,the phagocytosis functions of murine peritoneal macrophages interacted with soup and its nanoparticles were also investigated.The results showed that freshwater clam soup and its incidental nanoparticles did not show apparent toxicity to the four cell within the concentration of 15.63-500.00μg/mL.Further study found that murine peritoneal macrophages could engulf freshwater clam soup incidental nanoparticles.They did not influence the membrane potential and phagocytosis function of the normal peritoneal macrophages but prevented the cells from the membrane hyperpolarization,mitochondrial oxidative stress,and phagocytosis suppression induced by AAPH-induced oxidative stress.The antioxidant analysis revealed that freshwater clam soup incidental nanoparticles exerted FRAP and ABTS antioxidant activity,but not ORAC antioxidant activity.The research results provided a scientific basis for further research on the physiological function and related mechanism of freshwater clam soup and its incidental nanoparticles.