复方七芍降压片通过miR-214治疗高血压大鼠左心室肥厚实验研究

Experimental Study on the Treatment of Left Ventricular Hypertrophy in Hypertensive Rats with Compound Qishao Jiangya Tablets via miR-214

目的:从miR-214角度探讨复方七芍降压片对高血压大鼠血压以及左心室肥厚的影响。方法:采用无创大鼠尾动脉血压测定系统实时监测大鼠血压变化;采用超声心动仪测定大鼠心脏结构;观察大鼠心脏大体形态及心指数、左心室质量指数;采用苏木精-伊红染色法(HE)染色检测大鼠心肌组织病理情况;采用放射免疫法及酶联免疫吸附法(ELISA)检测大鼠心室肥厚血清学指标及心肌和血浆血管紧张素Ⅱ(Ang Ⅱ)表达变化;采用RT-PCR检测复方七芍降压片干预后肥大心肌细胞miR-214的变化情况。结果:血压结果显示复方七芍降压片能改善高血压大鼠血压;超声心动结果显示复方七芍降压片能改善高血压大鼠心脏功能;病理学结果显示复方七芍降压片能改善高血压大鼠心室肥厚情况;放射免疫法及ELISA结果显示复方七芍降压片能够降低大鼠血清型前胶原(PC Ⅲ)、层粘连蛋白(LN)、透明质酸(HA)及心肌和血浆AngⅡ表达,RT-PCR结果显示复方七芍降压片抑制肥大心肌细胞miR-214表达,以上结果差异均有统计学意义(P<0.05)。结论:复方七芍降压片可能通过抑制miR-214表达改善高血压大鼠的血压及心室肥厚水平。

Objective: To investigate the effects of Compound Qishao Jiangya Tablets on blood pressure and left ventricular hypertrophy in hypertensive rats via miR-214. Methods:The blood pressure changes of rats were monitored in real time by using a non-invasive tail artery blood pressure measurement system.The structure of rat heart was measured by echocardiography.Cardiac gross morphology,cardiac index and left ventricular mass index were observed.Hematoxylin eosin staining(HE) was used to detect the pathological changes of myocardium in rats. Radioimmunoassay and enzyme-linked immunosorbent assay(ELISA) were used to detect the serum indexes of ventricular hypertrophy and the expression of angiotensinⅡ(Ang Ⅱ) in myocardium and plasma of rats. RT-PCR was used to detect the changes of miR-214 in hypertrophic cardiomyocytes after the intervention of Compound Qishao Jiangya Tablets. Results: The results of blood pressure showed that Compound Qishao Jiangya Tablets could improve the blood pressure of hypertensive rats. The results of echocardiography showed that Compound Qishao Jiangya Tablets could improve the cardiac function of hypertensive rats. The pathological results showed that Compound Qishao Jiangya Tablets could improve the ventricular hypertrophy in hypertensive rats. The results of radioimmunoassay and ELISA showed that Compound Qishao Jiangya Tablets could reduce the expression of serum type procollagen(PC Ⅲ), laminin(LN), hyaluronic acid(HA) and Ang Ⅱ in myocardium and plasma of rats. The RT-PCR results showed that Compound Qishao Jiangya Tablets inhibited the expression of miR-214 in hypertrophic cardiomyocytes, and the difference of the above results was statistically significant(P<0.05). Conclusion: Compound Qishao Jiangya Tablets may improve blood pressure and ventricular hypertrophy in hypertensive rats by inhibiting the expression of miR-214.