基于网络药理学探讨丹参-檀香治疗冠心病的作用机制

Study on the Mechanism of Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum in Treating Coronary Heart Disease Based on Network Pharmacology

目的:应用网络药理学相应原理探讨丹参-檀香药对治疗冠心病(CHD)的作用机制。方法:在网络药理学的相应原理基础上,首先分别用“丹参”和“檀香”为关键词,在中药系统药理学数据库与分析平台(TCMSP)中获取其活性成分和相应的靶点蛋白,在数据库检索“冠心病”,筛选出潜在的、可治疗CHD的“丹参-檀香”药对的靶点,然后在DAVID数据库和Matescape数据库中进行GO分析、KEGG的富集分析。结果:通过使用TCMSP进行检索,可得到檀香的有效成分有3个,丹参的有效成分有65个。在韦恩图中,筛选出的CHD与药对共有靶点27个。KEGG和GO富集分析表明丹参-檀香药对改善CHD可能与脂质与动脉粥样硬化途径、流体切应力和动脉粥样硬化途径有关。分子对接结果表明丹参-檀香能够与CHD相关靶点紧密结合。分析表明,丹参-檀香配伍后可能存在针对CHD的协同作用。结论:丹参-檀香药对治疗CHD的过程涉及多种有效成分、靶点和通路,该结果可进一步为研究丹参-檀香药对治疗CHD的作用机制提供相应参考。

Objective:To explore the mechanism of Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum in treating coronary heart disease(CHD) by applying the corresponding principles of network pharmacology. Methods:On the basis of the corresponding principles of network pharmacology,the active components and corresponding target proteins were obtained from Traditional Chinese Medicine Systematic Pharmacology Database and Analysis Platform(TCMSP) with "Salviae Miltiorrhizae Radix et Rhizoma" and "Santali Albi Lignum" as the keywords respectively, and the "coronary heart disease" was searched in the database to screen out the potential targets of "Salvia sandalwood" drug pairs that can treat CHD, and then GO analysis was conducted in DAVID database and Matescape database Enrichment analysis of KEGG. Results:By using TCMSP to search,we can get 3 active components of Santali Albi Lignum and 65 active ingredients of Salviae Miltiorrhizae Radix et Rhizoma. In the Venn diagram,there are27 targets for CHD and drug pairs. The enrichment analysis of KEGG and GO indicated that the improvement of CHD by Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum may be related to lipid and atherosclerotic pathways,fluid shear stress and atherosclerotic pathways. The results of molecular docking showed that Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum could closely combine with CHD related targets. The analysis shows that there may be a synergistic effect against CHD after the compatibility of Salviae Miltiorrhizae Radix et Rhizoma and Santali Albi Lignum. Conclusion: The process of Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum in the treatment of CHD involves a variety of effective components, targets and pathways. The results can further provide a reference for the study of the mechanism of Salviae Miltiorrhizae Radix et Rhizoma-Santali Albi Lignum in the treatment of CHD.