lncRNA NONHSAT070341在宫颈癌中的差异表达及其对宫颈癌细胞生物学特性的影响

Differential expression of lncRNA NONHSAT070341 in cervical cancer and its effect on the biological characteristics of cervical cancer cells

目的:探究长链非编码RNA(long noncoding RNA,lncRNA)NONHSAT070341在宫颈癌组织及细胞系SiHa和Caski中的表达及其对宫颈癌细胞生物学功能如增殖、迁移、侵袭、凋亡及细胞周期分布的影响。方法:收集2021年06月至2022年06月于山西医科大学第二临床医学院妇产科行手术切除的处于不同宫颈病变阶段的患者90例,以30例同期因子宫肌瘤行子宫全切术的正常宫颈组织作为对照,通过实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)检测宫颈癌组织及细胞系中lncRNA NONHSAT070341的表达情况;在SiHa及Caski细胞中瞬时转染小干扰RNA(small interfering RNA,siRNA)干扰片段来靶向敲低lncRNA NONHSAT070341,并采用qRT-PCR验证转染效率;通过CCK-8和平板克隆形成实验检测细胞增殖能力,细胞周期实验检测对宫颈癌细胞周期分布的影响,划痕愈合实验评估细胞横向迁移能力,Transwell实验检测细胞纵向迁移及侵袭能力,流式细胞术分析细胞凋亡情况。结果:lncRNA NONHSAT070341在宫颈癌组织和细胞中高表达;NONHSAT070341 siRNA可降低SiHa和Caski细胞中NONHSAT070341的表达;敲低NONHSAT070341后,宫颈癌细胞的增殖、迁移、侵袭能力明显降低,细胞停滞在G 1期,影响了宫颈癌细胞周期的分布,但却增加了SiHa和Caski细胞的凋亡率。结论:NONHSAT070341可促进宫颈癌细胞的增殖、迁移及侵袭,改变了宫颈癌的生物学行为并有望成为诊断宫颈癌的一个新兴生物标志物。

Objective:To explore the expression of long noncoding RNA(lncRNA)NONHSAT070341 in cervical cancer tissues and cell lines SiHa and Caski and its effect on the biological functions of cervical cancer cells such as proliferation,migration,invasion,apoptosis and cell cycle distribution.Methods:A total of 90 patients with different stages of cervical lesions who underwent surgical resection in the department of gynecology and obstetrics,the Second Clinical Medical College of Shanxi Medical University from June 2021 to June 2022 were collected,and 30 cases of normal cervical tissue undergoing total hysterectomy due to uterine fibroids at the same time were used as control.The expression of lncRNA NONHSAT070341 in cervical cancer tissues and cell lines was detected by quantitative real-time polymerase chain reaction(qRT-PCR).The lncRNA NONHSAT070341 was knocked down by transient transfection of small interfering RNA(siRNA)fragment in SiHa and Caski cells,and the transfection efficiency was verified by qRT-PCR.The cell proliferation ability was detected by CCK-8 and plate colony formation assay.The cell cycle assay was used to detect the effect on the cycle distribution of cervical cancer cells.The scratch healing assay was used to evaluate the lateral migration ability of cells,and the Transwell assay was used to determine the longitudinal migration and invasion ability of cells.Flow cytometry analyzed cell apoptosis.Results:The lncRNA NONHSAT070341 was highly expressed in cervical cancer tissues and cells.NONHSAT070341 siRNA can reduce the expression of NONHSAT070341 in SiHa and Caski cells.After knocking down NONHSAT070341,the proliferation,migration and invasion abilities of cervical cancer cells were significantly reduced,and the cells were arrested in the G 1 phase,influencing the distribution of cervical cancer cell cycle,whereas the rate of apoptosis of SiHa and Caski cells were enhanced.Conclusion:NONHSAT070341 can promote the proliferation,migration and invasion of cervical cancer cells,change the biological behavior of cervical cancer and be expected to become an emerging biomarker for the diagnosis of cervical cancer.