自营养脱氮反应器快速启动影响因素研究

Study on Influencing Factors of Quickly Starting for the Autotrophic Nitrogen Removal Reactor

构建了3组自营养脱氮反应器,采用对比试验研究了接种污泥和pH等因素对自营养脱氮反应器启动效能的影响规律,解析了其作用原理。研究结果表明,接种1∶1比例厌氧氨氧化污泥和反硝化污泥的混合污泥,自营养脱氮反应器启动更快,并具有较好的脱氮效能,氨氮去除率稳定在90%以上;pH为7.6~8.0时对自营养脱氮反应器的启动影响较小,pH为8.5~8.9时表现出对厌氧氨氧化菌的抵制作用,亚硝酸盐积累现象明显,不能正常启动自营养脱氮反应器。分子生物学解析表明,不同接种污泥以及不同pH环境均会造成反应器中菌落结构的差异性变化,关键功能菌群相对丰度减小,会影响到自营养脱氮反应体系的平衡,反应器脱氮效能下降。

Three groups of autotrophic nitrogen removal reactors were constructed.The effects of inoculated sludge seeding and pH on the start-up efficiency of the reactors were studied by comparative experiments,and the action principles were analyzed.The research results show that autotrophic nitrogen removal starts faster when the mixed sludge is mixed with anaerobic ammonia oxidation and denitrifying sludge in the proportion of 1 to 1,and has better nitrogen removal efficiency with the stable ammonia nitrogen removal rate above 90%;The effect of pH on the startup speed of autotrophic nitrogen removal reactor is lower when pH is 7.6~8.0;When pH is 8.5~8.9,it has resistance to anaerobic ammonia oxidizing bacteria,nitrite accumulates obviously and the reactor will not start normally.Molecular biology analysis shows that both different inoculated sludge and pH environment will cause different changes in the community structure in the reactor.The reduction of the relative abundance of key functional bacteria will affect the balance of the autotrophic nitrogen removal reaction system and reduce the nitrogen removal efficiency of the reactor.