胰腺癌S100A4和Midkine表达研究

Expression of S100A4and Midkine inpancreaticcancer

目的:探讨钙离子结合蛋白S100A4和中期因子Midkine表达与胰腺癌临床病理特征的关系。方法:收集2010年2月至2020年2月胰腺癌手术标本87例及距瘤体3 cm的癌旁胰腺组织20例,应用EliVisionTMplus免疫组织化学染色2步法检测S100A4和Midkine在癌组织及癌旁胰腺组织中的表达。运用SPSS 22.0软件分析,S100A4和Midkine阳性率、临床病理特征等计数资料用[n(%)]表示,组间比较采用χ^(2)检验及相关性分析。P<0.05为差异有统计学意义。结果:癌组织S100A4和Midkine阳性率分别为73.6%(64/87)和71.3%(62/87),癌旁胰腺组织均无阳性表达,差异均有统计学意义(P<0.05)。S100A4和Midkine表达与性别、年龄、肿瘤部位无关(P>0.05)。在瘤体直径>3 cm、低分化等级、TNM分期(Ⅲ+Ⅳ)、有浸润转移患者中S100A4和Midkine表达均高于瘤体直径≤3 cm、(中+高)分化等级、TNM分期(Ⅰ+Ⅱ)、无浸润转移患者,二者表达呈正相关性(r=0.58)。结论:S100A4和Midkine与胰腺癌发生发展显著相关,二者共阳性表达预示胰腺癌分化差、易出现浸润转移。

Objective To investigate the relationship between the expression of calcium ion binding protein S100A4 and Midkine and the clinicopathologic features of pancreatic cancer.Methods From February 2010 to February 2020,87 pancreatic cancer surgical specimens and 20 paracarcinoma pancreatic tissues 3 cm away from the tumor were collected.The expression of S100A4 and Midkine in cancer tissues and paracarcinoma pancreatic tissues were detected by Elivision'plus immunohistochemical staining method.SPSS 22.0 software was used for analysis,S100A4 and Midkine positive rate,clinicopathological features and other count data were expressed by[n(%)],and comparison between groups was performed by X test and correlation analysis,P<0.05 was considered statistically significant.Results The positive rates of S100A4 and Midkine in cancer tissues were 73.6%(64/87)and 71.3%(62/87),respectively.There were no positive expressions in paracancer pancreas tissues,with statistical significance(P<0.05).The expressions of S100A4 and Midkine were not correlated with gender,age or tumor site(P>0.05).The expressions of S100A4 and Midkine in patients with tumor diameter>3 cm,low differentiation grade,TNM stage(III+IV),and invasive metastasis were higher than those in patients with tumor diameter≤3 cm,(medium+high)differentiation grade,TNM stage(I+II),and no invasive metastasis,and the expressions were positively correlated(r=0.58).Conclusion S100A4 and Midkine were significantly correlated with the occurrence and development of pancreatic cancer,and both positive expressions indicated poor differentiation and easy invasion and metastasis of pancreatic cancer.