牛筋果茎中一个新的异戊烯基异黄酮类化合物

A New Prenylated Isoflavone from the Stems of Harrisonia perforata

综合运用硅胶柱色谱、ODS柱色谱、SephadexLH-20凝胶柱色谱以及制备型高效液相色谱(HPLC)等色谱分离手段,对苦木科牛筋果属植物牛筋果Harrisoniaperforata茎中的化学成分进行了研究,从其茎的95%乙醇提取物中分离得到了8个异戊烯基异黄酮类化合物,采用多种波谱鉴定方法确定了这些分离得到化合物的化学结构,其中化合物1为1个新的异戊烯基异黄酮类化合物,化合物2~8为从牛筋果属植物中分离得到的化合物.化合物1~8体外抑制肿瘤细胞生长的活性评价结果表明,它们对五种肿瘤细胞株(HL-60、A549、SMMC-7721、MCF-7和SW480)均具有较为显著的体外生长抑制活性,抑制肿瘤细胞生长的活性的IC50范围为(0.38±0.03)~(31.68±0.21)μmol/L,抑制肿瘤细胞生长的活性与抗肿瘤阳性对照药顺铂的抑制活性相当.

A new prenylated isoflavone,harriperforine(1),as well as seven known analogues,was isolated and characterized from the stems of Harrisonia perforata.Their chemical structures were unambiguously elucidated based on spectroscopic analyses.All known compounds 2~8 were isolated from the genus Harrisonia for the first time.The growth inhibitory activities against five cancer cell lines(HL-60,A549,SMMC-7721,MCF-7 and SW480)of compounds 1~8 were evaluated.As a result,compounds 1~8 displayed remarkable inhibitory activities with IC_(50)values ranging from(0.38±0.03)μmol/L to(31.68±0.21)μmol/L,which were comparable to those of cisplatin.