分散固相萃取与GC-MS联用检测咖啡豆及速溶咖啡中糠醛类化合物

Determination of Furfural Compounds in Coffee Beans and Instant Coffee by GC-MS Combined with Dispersive Solid Phase Extraction

目的:建立分散固相萃取与气相色谱-质谱法(GC-MS)联用检测咖啡豆及速溶咖啡中糠醛类化合物的分析方法。方法:样品先经含有Na2CO3的50%乙醇-水溶液超声溶解提取,再经乙腈提取后加入MgSO4、C18及GCB分散固相萃取净化,最后经PTFE针式过滤器过滤后进GC-MS分析。结果:在优化了样品提取、分散萃取及基质效应等实验条件下,3种糠醛类化合物在质量浓度0.1~20.0μg/mL范围内相关系数均大于0.9935,RSD在2.11%~3.84%之间,加标回收率在89.7%~103.5%之间。结论:该方法简便、准确、灵敏、有机溶剂消耗少,能降低基质影响,完全满足咖啡豆和速溶咖啡中糠醛类化合物的同时定性和定量分析。

Object:A method was developed for the determination of furfural compounds in coffee beans and instant coffee by GC-MS with dispersive solid phase extraction(DSPE).Method:The sample was dissolved and extracted by ultrasonic with 50%ethanol-water solution containing Na,cO3,then extracted by acetonitrile and purified by DSPE with MgSO4,Cs and GCB,at last the purifying solution was determined by GC-MS analysis after the PTFE needle filter.Result:The conditions of sample extraction,dispersion extraction and matrix effect were optimized.The correlation coefficients of the 3 furfural compounds were more than 0.9935 in the range of 0.1~20.0μg/mL,and the RSD was between 2.11%and 3.84%,the recoveries were between 89.7%and 103.5%.Conclusion:The method is simple,accurate,sensitive,less consumption of organic solvent,less influence of matrix,and suitable for the qualitative and quantitative analysis of furfural compounds in coffee beans and instant coffee.