基于生物信息学分析CILP2基因在结直肠癌中表达及其与预后的相关性

CILP2 gene expression in colorectal cancer and its correlation to the prognosis based on bioinformatics

目的:探究CILP2基因在结直肠癌中的表达,分析CILP2基因的表达与临床病理特征、预后的关系,探索CILP2基因在结直肠癌发生发展中的作用机制。方法:从癌症基因组图谱(TCGA)数据库官方网站上下载结直肠癌的RNA-seq数据及患者的临床病理信息,提取CILP2在结直肠癌组织和正常组织样本中的表达量数据,使用Mann-Whitney U检验分析两组之间的表达差异。根据CILP2在结直肠癌样本中表达水平的中位数,将结直肠癌患者划分为高、低表达两组,使用χ^(2)检验分析CILP2表达与患者临床病理特征的联系,利用Kaplan-Meier法分析CILP2表达与患者预后的相关性。使用单因素和多因素Cox回归分析CILP2表达与结直肠癌患者临床预后相关性。利用基因富集分析(GSEA)探索CILP2在结直肠癌中参与的信号途径。结果:结直肠癌样本中CILP2表达水平显著高于正常组织中CILP2的表达水平,差异具有统计学意义(P<0.05);CILP2的表达与患者的原发肿瘤分期(P=0.014)和淋巴结转移分期(P=0.016)显著相关。CILP2高表达组患者的总体生存率显著低于低表达组患者,且差异具有统计学意义(P=0.009)。多因素Cox回归分析显示,CILP2可以作为结直肠癌患者的独立预后因素(HR=1.288,95%CI:1.058~1.569,P=0.012)。GSEA富集分析结果提示,CILP2主要参与WNT信号通路、刺猬信号通路、肿瘤相关的通路等通路。结论:CILP2在结直肠癌中表达上调,参与多种癌症信号通路,可作为结直肠癌患者的独立预后因素,是结直肠癌新型分子标志物。

Objective:To explore the expression of CILP2 gene in colorectal cancer,analyze the relationship between CILP2 expression and clinicopathological characteristics and prognosis,and explore the mechanism of CILP2 role in the development and progression of colorectal cancer.Methods:RNA-seq data and clinicopathological information of colorectal cancer patients were downloaded from the official website of the Cancer Genome Atlas(TCGA)database,and CILP2 expression data were extracted from colorectal cancer tissues and normal tissue samples.Mann-Whitney U test was used to analyze the expression differences between the two groups.Colorectal cancer patients were divided into high and low expression groups based on the median level of CILP2 expression in the colorectal cancer sample,and the correlation between CILP2 expression and clinicopathological characteristics was analyzed using the Chi-square test,and the correlation between CILP2 expression and patient prognosis was analyzed using the Kaplan-Meier method.Prognostic value was determined by univariate and multivariate Cox regression analysis.Gene Set Enrichment Analysis(GSEA)was adopted to explore the signaling pathway involved in CILP2.Results:CILP2 expression in colorectal cancer tissues was significantly higher than that in normal tissues(P<0.05),CILP2 expression was significantly correlated with primary tumor stage(P=0.014)and lymph node metastasis stage(P=0.016).Overall survival rate of patients in the high-expression group was significantly lower than that in the low-expression group(P=0.009).Multivariate Cox regression analysis showed that CILP2 was an independent prognostic factor for colorectal cancer patients(HR=1.288,95%CI:1.058-1.569,P=0.012).GSEA results indicated that CILP2 was mainly involved in WNT signaling pathway,hedgehog signaling pathway,and tumor-related pathway.Conclusion:CILP2 is up-regulated in colorectal cancer,and is involved in multiple cancer signaling pathways.CILP2 is an independent prognostic factor for colorectal cancer patients and is a novel molecular marker for colorectal cancer.