壮骨强肌方对去势大鼠骨骼肌形态和功能的影响

Effects of Zhuanggu Qiangji Decoction on skeletal muscle morphology and function in ovariectomized rats

目的探讨壮骨强肌方对去卵巢模型大鼠的骨骼肌形态及功能的影响。方法32只3月龄雌性SD大鼠常规饲养3个月后,随机分为假手术组8只和模型组24只,假手术组切除卵巢周围部分脂肪组织,模型组则切除双侧卵巢;术后喂养12周后,模型组再等分为3组,包括模型对照组、壮骨强肌方组和阿仑膦酸钠组,每组再抽取2只大鼠验证去卵巢模型造模成功。最后按照等效剂量换算公式予以各组灌胃处理,连续干预12周。灌胃结束后,测定大鼠的四肢抓力;取腓肠肌和比目鱼肌称重并计算骨骼肌质量指数,行HE染色观察肌纤维形态结构、测量肌纤维横截面积。结果假手术组、壮骨强肌方组和阿仑膦酸钠组大鼠的四肢抓力、腓肠肌指数、小腿三头肌指数均明显高于模型组(P<0.05);假手术组和壮骨强肌方组的腓肠肌肌纤维平均横截面积均明显高于模型组(P<0.05),而阿仑膦酸钠组相对于模型组则无统计学意义(P>0.05);假手术组、壮骨强肌方组和阿仑膦酸钠组3者之间的差异均无统计学意义(P>0.05),在HE染色镜下3组大鼠的骨骼肌纤维形态结构均好于模型对照组。结论壮骨强肌方能够明显改善去卵巢模型大鼠骨骼肌的形态和功能。

Objective To investigate the effects of Zhuanggu Qiangji Decoction on skeletal muscle morphology and function in ovariectomized rats.Methods Thirty-two 3-month-old female Sprague-Dawley(SD)rats were routinely fed for three months,and then randomly divided into sham operation group(n=8)and model group(n=24).In the sham operation group,part of the adipose tissue around the ovaries was removed,while the rats in the model group underwent bilateral ovariectomy.After 12 weeks of postoperative feeding,the model group was equally divided into three groups,including model control group,Zhuanggu Qiangji decoction group and allenphosphate sodium group.Two rats were selected from each group to verify the success of ovariectomy model.Finally,according to the equivalent dose conversion formula,the rats in each group were treated by gavage for 12 weeks.After the end of gavage,the limb grip strength of the rats was measured.The gastrocnemius and soleus muscles were weighed and the skeletal muscle mass index was calculated.The morphological structure of muscle fibers was observed by HE staining,and the cross-sectional area of muscle fibers was measured.Results The limb grip strength,gastrocnemius index and triceps calves index of rats in the sham operation group,Zhuanggu Qiangji decoction group and alendronate sodium group were significantly higher than those in the model group(P<0.05).The mean cross-sectional area of gastrocnemius muscle fiber in the sham operation group and the Zhuanggu Qiangji decoction group was significantly higher than that in the model group(P<0.05),while there was no significant difference between the alendronate sodium group and the model group(P>0.05).Besides,there was no significant difference among the sham operation group,Zhuanggu Qiangji decoction group and alendronate sodium group(P>0.05).The morphological structure of skeletal muscle fibers in the three groups was also better than that in the model control group under HE staining.Conclusion Zhuanggu Qiangji decoction can significantly improve the morphology and function of skeletal muscle in ovariectomized rats.