赣南脐橙黄龙病植株根际土壤微生物多样性研究

Microbial diversity in rhizosphere soil of navel orange infected with citrus Huanglongbing in southern Jiangxi province

【目的】分析赣南脐橙黄龙病患病植株和健康植株根际土壤微生物多样性和群落组成,为黄龙病新型生态防控提供参考依据。【方法】以赣南脐橙黄龙病典型患病植株、疑似患病植株、健康植株根际土壤和裸地土壤为研究对象,利用高通量测序技术比较分析样品间微生物多样性和群落结构特征,并探讨土壤理化性质与微生物群落的关系。【结果】赣南脐橙患病植株根际土壤细菌群落的丰富度和多样性均高于健康植株根际土壤,其真菌群落的多样性也高于健康植株根际土壤,但真菌群落丰富度低于健康植株根际土壤;脐橙患病植株与健康植株根际土壤微生物群落组成相似,但相对丰度存在差异;在门水平上,患病植株根际土壤中绿弯菌门、酸杆菌门等菌门增加,而放线菌门、担子菌门等菌门有所减少;在属水平上,患病植株根际土壤中芽杆孢菌属、篮状菌属等有益菌属减少,而镰刀菌属、曲霉属等致病菌属则有所增加;患病植株根际土壤中全氮、全磷含量均低于健康植株根际土壤,而全碳含量高于健康植株根际土壤,全磷是影响样本根际土壤微生物多样性和群落组成变化的主要因素。【结论】赣南脐橙感染黄龙病后,其根际土壤理化性质发生了明显变化,土壤微生物多样性和群落组成也有所改变,这些差异在一定程度上体现了黄龙病对脐橙根际微生态的影响。

【Objective】The study aimed to analyze the microbial diversity and community composition in the rhizospheric soil of the navel or-ange with citrus Huanglongbing to provide the new clue for ecological control of citrus Huanglongbing.【Method】The bacterial 16S rRNA re-gion and fungal ITS region from the rhizosphere soils of diseased plants,healthy plants and bare land were sequenced and analyzed to evalu-ate the microbial community structure and diversity.Moreover,the physicochemical properties of four soil samples were also measured to de-termine the relationship between microbial community and soil physicochemical properties.【Result】The richness and diversity of bacterial community in rhizosphere soil of diseased plants were higher than that in the healthy plants,and the diversity of the fungal community in rhi-zosphere soil of diseased plants were also higher than that in the healthy plants,which the richness was lower than that of healthy plants.The microbial phyla found in diseased and healthy plants were similar on composition but significantly differed on relative abundance.Choroflexi and Acidobacteria had a higher abundance in the rhizosphere soil of diseased plants than that of healthy plants,whereas Actinobacteria and Basidiomycota had a lower abundance.At the genus level,the abundance of beneficial microorganisms,such as Bacillus and Talaromyces were lower in the rhizosphere soil of diseased plants than that of healthy plants,whereas the abundance of pathogen such as Fusarium and As-pergillus were higher.The contents of total phosphorus and total nitrogen were significantly lower in the rhizosphere soil of diseased plants than those of healthy plants,whereas the total carbon content was increased in diseased soil,and total phosphors had a significant impact on rhizo-sphere soil microbial community.【Conclusion】After navel oranges were infected with citrus Huanglongbing,the rhizosphere soil physical and chemical properties changed significantly,and the microbial diversity and community composition in rhizosphere soil of diseased plants also changed.To a certain extent,these differences reflect the effect of citrus Huanglongbing on the rhizosphere microecology of navel oranges.