银屑病小鼠行为学、血液及皮肤组织病理学指标检测与分析

Analysis of behavioral,serum,and skin histology tests in experimental psoriasis mice

目的探讨银屑病小鼠行为学、血液及皮肤组织病理学指标的变化。方法将BALB/c小鼠,随机分为对照(Control)组、咪喹莫特诱导银屑病模型(imiquimod-induced psoriasis model,IMQ)组、焦虑复合咪喹莫特诱导银屑病模型(emotional stress complex imiquimod-induced psoriasis model,ES+IMQ)组,随后观察各组小鼠旷场实验、Y迷宫实验、血清P物质及皮肤组织病理学指标变化。结果旷场实验,与对照组比较,总区域内IMQ组和ES+IMQ组总路程缩短,平均速度降低,停留时间延长;中心区域内路程、停留时间缩短等;边缘区域内路程、停留时间相对延长等。Y迷宫实验,与对照组比较,IMQ组和ES+IMQ组新异臂进入次数百分比、新异臂停留时间百分比下降等。血清P物质,与对照组比较,IMQ组小鼠血清P物质浓度升高、ES+IMQ组小鼠血清P物质浓度降低,且两个模型组有统计学差异。皮肤组织病理学结果显示,与对照组比较,IMQ组和ES+IMQ组均可出现银屑病样皮肤组织病理学改变,Baker评分均有统计学差异,但两个模型组没有统计学差异。结论本实验条件下IMQ组和ES+IMQ组小鼠均可出现自发活动能力降低、产生焦虑样行为,小鼠认知能力受损,对血清P物质水平有影响,具有银屑病样皮肤组织病理学变化;其中ES+IMQ组比IMQ组焦虑样表现较明显,同血清SP水平变化程度成反比,其余指标无明显差异。

Objective To investigate behavioral,blood,and skin histopathological indexes in psoriasis mice.Methods BALB/c mice were randomly divided into a control group,imiquimod-induced psoriasis model group(IMQ),and emotional stress complex imiquimod-induced psoriasis model group(ES+IMQ)and observed in an open field test(OFT)and Y maze test,and tested for serum substance P(SP)and skin histopathological indexes.Results In the OFT,Compared with the control group,both the IMQ group and ES+IMQ group covered shorter total distances,with decreased average velocity and extended residence time in the total areas and they had a shorter residence time and distance in the central area and extended distance and residence time in the edge area.In the Y maze test,the percentage of entries and percentage of residence time in the new arm decreased in both IMQ and ES+IMQ groups compared with control group.The serum SP concentration was increased in the IMQ group and decreased in the ES+IMQ group compared with the control group,and there was a statistical difference between the two model groups.Psoriasis-like skin histopathological changes were observed in both IMQ and ES+IMQ groups.The Baker score of changes were statistically different,but there were no statistical differences between the IMQ and ES+IMQ groups.Conclusions Under the experimental conditions,both IMQ and ES+IMQ group s showed decreased spontaneous activity,anxiety-like behavior,and impaired cognitive ability,which had an effect on serum SP levels,and they showed psoriasis-like skin histopathological changes.The ES+IMQ group showed more anxiety than the IMQ group,which was inversely proportional to the difference in serum SP levels,and the other indicators showed no significant differences.