黄腐酚逆转B16-F10黑色素瘤细胞恶性表型

Xanthohumol Reverses Malignant Phenotype of B16-F10 Melanoma Cells

本研究从体外增殖、细胞周期分布、克隆形成和迁移能力、黑色素合成以及糖酵解水平等考察了黄腐酚(XN)对B16-F10高转移潜能小鼠黑色素瘤细胞恶性表型的影响。结果表明,XN明显抑制B16-F10细胞增殖、克隆形成和体外迁移,阻滞细胞周期于G0/G1期,并上调细胞黑色素合成水平。同时发现XN下调B16-F10细胞的葡萄糖摄取,降低乳酸脱氢酶和己糖激酶活性及NAD^(+)/NADH比率,下调缺氧诱导因子1(HIF-1α)和沉默信息调节因子2相关酶1(SIRT1)表达。另外,XN明显延长荷B16-F10黑色素瘤小鼠生存期。总之,本研究表明XN逆转B16-F10黑色素瘤恶性表型,并发挥抗肿瘤增殖和转移活性,或与其调控肿瘤糖代谢效应相关。

In this study,the effects of xanthohumol(XN)on the malignant phenotype of B16-F10 high metastatic potential mouse melanoma cells are investigated from the aspects of proliferation,cell cycle,clone formation ability,migration ability,melanin contents and glycolysis level in vitro.The resuts show that,XN significantly inhibits the proliferation,colony formation ability and migration ability of B16-F10 cells,arrests the cell cycle at the G0/G1 phase,and up-regulates the melanin contents.In addition,XN down-regulates glucose uptake of B16-F10 cells,reduces the activities of lactate dehydrogenase(LDH)and hexokinase(HK)as well as the ratio of NAD^(+)/NADH,decreases the level of hypoxia inducible factor-1(HIF-1 alpha)and sirtuin1(SIRT1).It is further confirmed that XN significantly prolongs the survival time of B16-F10 melanoma mice.In conclusion,XN can reverse the malignant phenotype of B16-F10 melanoma cells and exert anti-tumor proliferation and metastasis activity,which may be related to regulating tumor glucose metabolism.