14种沙棘果实中氨基酸组成的主成分分析与综合评价

Principal Component Analysis and Cluster Analysis of Amino Acids in Seabuckthorn Fruit

【目的】研究不同沙棘优株果实中氨基酸含量和组成成分,为沙棘良种选育提供理论依据。【方法】以阿勒泰地区14种沙棘优株为研究对象,以新疆主栽品种深秋红为对照,采用氨基酸自动分析仪快速检测氨基酸种类及含量,运用相关性分析、主成分分析法及聚类分析法对沙棘果实中氨基酸含量进行综合评价。【结果】14种沙棘果实氨基酸指标之间存在相对独立性和密切相关性,其中缬氨酸和异亮氨酸极显著相关(r=0.982,P<0.01)。14种沙棘果实综合排名前5位的依次为BT-06-04、QH-03-02、BT-08-02、HK-01-01、拟QH-03-02,排名居后的为:深秋红、ANJR、HH-09-01。主成分分析提取3个主成分,累计方差贡献率为88.296%,得到综合评价模型:F=0.802F_(1)+0.122F_(2)+0.076F_(3),较好地反映沙棘果实氨基酸的综合信息。14种沙棘果实分为3类,该聚类结果与主成分分析结果一致,较好地反映出沙棘果实不同种质间的差异性。【结论】BT-06-04、QH-03-02、BT-08-02的氨基酸含量最高,可作为沙棘良种选育的育种材料。

【Objective】In order to study the differences of amino acid content and composition of different seabuckthorn varieties,15 seabuckthorn varieties in Qinghe County were studied,and the amino acid types and content were detected quickly by amino acid automatic analyzer.【Methods】The amino acid contents of 15 Seabuckthorn fruits were comprehensively evaluated by correlation,principal component analysis and cluster analysis.【Results】There were relative independence and close correlation among 15 quality indexes of seabuckthorn fruit;According to the comprehensive score of principal factors of principal component analysis,the top 5 seabuckthorn varieties ranked by 15 seabuckthorn varieties were BT-06-04,QH-03-02,BT-08-02,HK-01-01 and proposed QH-03-03,followed by late autumn red,anjr and HH-09-01.【Conclusion】Three principal components were extracted by principal component analysis,and the cumulative variance contribution rate was 88.296%.A comprehensive evaluation model was obtained:F=0.802 F_(1)+0.122 F_(2)+0.076 F_(3),which better reflected the comprehensive information of amino acids in seabuckthorn fruit.Fifteen Seabuckthorn fruits were divided into three categories by systematic cluster analysis.The cluster results were consistent with the results of principal component analysis,which better reflected the differences among different germplasm of seabuckthorn fruits.