临床分离粪肠球菌的毒力基因和耐药基因检测及其耐药性研究

Virulence genes,drug resistance genes and drug resistance of clinically isolated Enterococcus faecalis

分离女性阴道中的粪肠球菌(Enterococcus faecalis),通过PCR和Kirby-Bauer(K-B)纸片扩散法探究其毒力基因、耐药基因及其耐药表型,为揭示粪肠球菌感染机制及其临床治疗提供科学依据。结果表明:从157个样品中共分离出22株粪肠球菌;在这些粪肠球菌中:毒力基因检出率为efaA(100%)、asa1(90.9%)、cylA(90.9%)、fsr(90.9%)、cpd(90.9%)、acm(86.4%)、gelE(81.8%)、esp(68.2%)、ace(54.5%)和hyl(0);耐药基因的检出率为vanA(0)、vanB(0)、vanC(18.2%)、aac(40.9%)、ant(6)-1(59.1%)、ermB(68.2%)、mefA(54.5%)、tetM(72.2%)和tem(81.8%);药敏结果显示粪肠球菌对万古霉素、呋喃妥因、利奈唑胺、替考拉宁敏感;对其他抗菌药物具不同程度的耐药。由此得出,阴道易受粪肠球菌感染,体外实验支持糖肽类抗菌药物可用于粪肠球菌感染的临床治疗。

To reveal the pathogenesis of Enterococcus faecalis and provide scientific basis for clinical treatment,several strains of E.faecalis were isolated from female vagina and their virulence genes,resistance genes,and drug resistance were detected by using PCR and Kirby Bauer(K-B)disk diffusion.The results showed that 22 strains of E.faecalis were isolated from 157 samples.Of the 22 strains isolated,efaA was the most commonly found virulence gene(100%),followed by asa1(90.9%),cylA(90.9%),fsr(90.9%),cpd(90.9%),acm(86.4%),gelE(81.8%),esp(68.2%),ace(54.5%),and hyl(0),and the drug resistance genes detected were vanA(0),vanB(0),vanC(18.2%),aac(6')/aph(2')(40.9%),ant(6)-1(59.1%),ermB(68.2%),mefA(54.5%),tetM(72.2%),and tem(81.8%).Antibiotic susceptibility testing by the K-B disk diffusion method showed that the isolates were sensitive to vancomycin,nitrofurantoin,linezolid and koalaranin,but resistant to other antibiotics in different degrees.To sum up,vaginas are susceptible to E.faecalis infection,and experiment in vitro indicated that glycopeptide antibiotics were promising in the clinical treatment choice for E.faecalis infection.