韭菜提取物对胰脂肪酶抑制作用的研究

Study on the inhibitory effect of leek extracts on pancreatic lipase

用乙醇提取韭菜粉末得到韭菜乙醇提取物,然后以正己烷、二氯甲烷、乙酸乙酯、正丁醇为萃取液,得到不同有机溶剂的提取物,研究其对胰脂肪酶的抑制作用,并与奥利司他的抑制作用进行比较。结果表明:不同溶剂提取物对胰脂肪酶的抑制活性强弱依次为乙酸乙酯>正己烷>二氯甲烷>正丁醇;随着不同溶剂提取物质量浓度的增大,抑制率越大;抑制胰脂肪酶的最佳反应条件为pH 7.5、反应时间15 min;乙酸乙酯提取物对胰脂肪酶的抑制为非竞争抑制类型,不如奥利司他的抑制作用强,但是可以作为良好的抑制胰脂肪酶的蔬菜原料。

Ethanol extract of leek was obtained by using ethanol to extract leek powder.Then,n-hex­ane,dichloromethane,ethyl acetate and n-butanol were used as the extraction solution to obtain the ex­tracts of different organic solvents,and their inhibition on pancreatic lipase was studied and compared with that of orlistat.The results showed that the inhibitory activity of different solvent extracts on pancre­atic lipase was in the order of ethyl acetate>n-hexane>dichloromethane>n-butanol,and the inhibition rate increased with the increase of the mass concentration of different solvent extracts.The optimal reac­tion conditions for inhibiting pancreatic lipase were pH 7.5,reaction time 15 min.The inhibition of ethyl acetate extract on pancreatic lipase was non-competitive and was not as strong as that of orlistat,but it could be used as a good vegetable material for inhibiting pancreatic lipase.