葛根素对绝经后骨质疏松大鼠不同部位骨骼的抗骨质疏松作用差异的研究

The difference of anti-osteoporosis effect of Puerarin on different parts of bone in postmenopausal osteoporosis rats

目的本文拟通过动物实验研究葛根素对大鼠骨密度的影响,探讨葛根素对不同部位骨骼抗骨质疏松作用的差异。方法对24只雌性SD大鼠行双侧卵巢切除术,构建大鼠绝经后骨质疏松模型。采用葛根素处理大鼠后,分别于第2、4、8、12周采用双能X射线吸收法(DXA)检测其各部位骨骼骨密度随时间发生的变化,并对比不同部位骨骼骨密度、骨矿含量的差异,同时通过组织切片观察葛根素对骨组织微结构的影响。结果葛根素应用后的第8周疗效最为显著,经葛根素处理后股骨远端、腰椎等松质骨的骨密度、骨矿含量显著提高,且骨小梁更粗、数量更多,而股骨中段等皮质骨的骨密度、骨矿含量变化则不大。结论葛根素对绝经后骨质疏松大鼠松质骨的抗骨质疏松作用优于皮质骨。

Objective This study aims to investigate the effect of Puerarin on bone mineral density in rats through animal experiments,and explore the diversity of anti-osteoporosis effect of puerarin in different parts of bones.Methods Bilateral ovariectomy was performed on 24 female SD rats to establish the postmenopausal osteoporosis model.After the rats were treated with puerarin,the changes of bone mineral density(BMD)were detected by dual energy X-ray absorption(DXA)in the 2nd,4th,8th and 12th weeks.The differences of BMD and bone mineral content(BMC)in different parts were compared.The effect of Puerarin on the microstructure of bone tissue was evaluated by observing tissue sections.Results The effect of Puerarin was the most significant in the 8th week since it was applied.After Puerarin Treatment,the BMD and BMC of cancellous bone such as distal femur and lumbar spine increased significantly,and bone trabeculae of treatment group were thicker and more in quantity,while there was little change in cortical bone such as middle femur.Conclusion Puerarin has a stronger anti-osteoporosis effect on cancellous bone than cortical bone in postmenopausal osteoporosis rats.