β-catenin、STT3A和PD-L1在胃癌组织中的表达及与病理特征和预后的相关性

Expression of β-catenin,STT3A and PD-L1 in gastric cancer and correlation with pathological festures and prognosis

目的:探讨β-catenin、STT3A及PD-L1相关蛋白在胃癌组织中的表达相关性及其与病理特征和预后相关性。方法:选取2020年02月至2021年10月63例胃癌组织及癌旁组织蜡块标本,通过免疫组化检测胃癌及癌旁组织中β-catenin、STT3A及PD-L1相关蛋白的表达情况,探讨其与临床病理参数之间的关系,并分析β-catenin、STT3A及PD-L1三者之间的相关性。通过Western blot检测胃癌细胞中的β-catenin、STT3A及PD-L1蛋白的表达情况,探讨稳定PD-L1表达糖基化修饰的可能影响因素,为胃癌免疫检查点抑制剂疗法提供新思路。结果:胃癌组织中β-catenin、STT3A阳性表达率高于癌旁组织(P<0.05),与肿瘤分化程度、肿瘤直径、淋巴结转移、临床TNM分期有关(P<0.05),但与年龄、性别均无显著相关性(P>0.05)。胃癌组织中PD-L1阳性表达率高于癌旁组织(P<0.05),与肿瘤分化程度、临床TNM分期有关(P<0.05),而与年龄、性别、肿瘤直径、淋巴结转移无显著相关性(P>0.05)。在胃癌组织中,β-catenin与STT3A、PD-L1的表达呈正相关(P<0.05),PD-L1与STT3A的表达呈正相关(P<0.05)。结论:胃癌组织中β-catenin、STT3A、PD-L1三种蛋白的表达均高于癌旁组织,且与肿瘤分化程度、临床TNM分期有关,因此三种蛋白可能与肿瘤的预后相关。此外,β-catenin、STT3A、PD-L1三者之间的的表达呈相关性,结果表明胃癌组织中可能存在β-catenin调控STT3A从而调节PD-L1的糖基化过程,从而影响PD-L1稳定性的表达。

Objective:To investigate the expression ofβ-catenin,STT3A and PD-L1 related proteins in gastric cancer and their correlation with pathological features and prognosis.Methods:The expression ofβ-catenin,STT3A and PD-L1 related proteins in gastric cancer and adjacent tissues were detected by immunohistochemistry from 63 cases of gastric cancer tissues and adjacent tissues from February 2020 to October 2021,and the relationship between the expression and clinicopathological parameters was investigated.The correlation among β-catenin,STT3A and PD-L1 was analyzed.The expressions of β-catenin,STT3A and PD-L1 proteins in gastric cancer cells were detected by Western blot.The possible influencing factors of glycosylation modification of stable PD-L1 expression were explored,to provide a new idea for immune checkpoint inhibitor therapy of gastric cancer. Results: The positive expression rates of β-catenin and STT3A in gastric cancer tissues were higher than those in adjacent tissues( P <0.05),which were related to tumor differentiation,tumor diameter,lymph node metastasis and clinical TNM stage( P <0.05),but had no significant correlation with age and gender( P >0.05).The positive expression rate of PD-L1 in gastric cancer tissues was higher than that in adjacent tissues( P <0.05),which was related to the degree of tumor differentiation and clinical TNM stage( P <0.05),but had no significant correlation with age,gender,tumor diameter and lymph node metastasis( P >0.05).In gastric cancer tissues,β-catenin was positively correlated with the expression of STT3A and PD-L1( P <0.05),and PD-L1 was positively correlated with the expression of STT3A( P <0.05). Conclusion: The expression levels of β-catenin,STT3A and PD-L1 proteins in gastric cancer are higher than those in the paracancer,and are related to the degree of tumor differentiation and clinical TNM stage,so the three proteins may be related to the prognosis of tumor.In addition,the expressions of β-catenin,STT3A and PD-L1 were correlated.The results indicated that β-catenin may regulate STT3A and thus regulate the glycosylation process of PD-L1 in gastric cancer tissues,thus affecting the stable expression of PD-L1.